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Invitrogen

PRDX1 Polyclonal Antibody

Name: PRDX1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA3750
Price: 474.0 USD
Availability: InStock

Advanced Verification

2 Published Figures

5 References

View all (16) PRDX1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

PRDX1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

PRDX1 Antibody (PA3-750) in ICC/IF

Immunofluorescence analysis of Peroxiredoxin 1 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRDX1 Rabbit Polyclonal (Product # PA3-750) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

Product Image Gallery

PRDX1 Antibody — Figure 5 Validation of expression profiling of proteins by Western blotting. Four of the identified proteins, PRDX1 ( A ), GLRX3 ( B ), ENO1 ( C ), and CASP1 ( D ), responding to AGE and/or FruArg treatment in LPS-stimulated BV-2 cells were validated using Western blotting. Protein intensities were quantified by Image J software, normalized to beta-actin, and expressed as percentage of untreated controls. Data are means +- SEM (n>=5); #, P<0.05, untreated vs. LPS; *, P<0.05, LPS vs. LPS+AGE; **, P<0.05, LPS vs. LPS+FruArg. {TM}

Product Details

PA3-750

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

1:250

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues L(103) V S D P K R T I A Q D(114) of human Peroxiredoxin 1.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2168166

Product Specific Information

PA3-750 detects Peroxiredoxin 1 protein in human, rat and mouse samples. This antibody is specific for Prx 1 and shows no cross-reactivity with other Prx isoforms.

PA3-750 has successfully been used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~26 kDa protein representing Prx 1 in human PC3 cell lysate.

The PA3-750 immunizing peptide corresponds to amino acid residues 103-114 from human Prx 1.

Target Information

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration.

eLife

Cho E,Che X,Ang MJ,Cheon S,Lee J,Kim KS,Lee CH,Lee SY,Yang HY,Moon C,Park C,Choi JY,Lee TH

PA3-750 was used in Western Blotting to show that Prdx5 is involved in osteoclast and osteoblast differentiation, resulting in osteoporotic phenotypes in Prdx5 knockout (Prdx5Ko) male mice.

Fri Feb 03 00:00:00 UTC 2023

Mitochondrial CoQ deficiency is a common driver of mitochondrial oxidants and insulin resistance.

eLife

Fazakerley DJ,Chaudhuri R,Yang P,Maghzal GJ,Thomas KC,Krycer JR,Humphrey SJ,Parker BL,Fisher-Wellman KH,Meoli CC,Hoffman NJ,Diskin C,Burchfield JG,Cowley MJ,Kaplan W,Modrusan Z,Kolumam G,Yang JY,Chen DL,Samocha-Bonet D,Greenfield JR,Hoehn KL,Stocker R,James DE

Published figure using PRDX1 polyclonal antibody (Product # PA3-750) in Western Blot

Tue Feb 06 00:00:00 UTC 2018

Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells.

PloS one

Zhou H,Qu Z,Mossine VV,Nknolise DL,Li J,Chen Z,Cheng J,Greenlief CM,Mawhinney TP,Brown PN,Fritsche KL,Hannink M,Lubahn DB,Sun GY,Gu Z

PA3-750 was used in western blot to study the effects of aged garlic extract and its FruArg component by proteomic analysis and lipopolysaccharide-induced neuroinflammatory response in microglial cells

Tue Jan 26 00:00:00 UTC 2016

Proteomic profiling identifies cyclooxygenase-2-independent global proteomic changes by celecoxib in colorectal cancer cells.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology

Lou J,Fatima N,Xiao Z,Stauffer S,Smythers G,Greenwald P,Ali IU

PA3-750 was used in western blot to find new celecoxib-mediated markers in the COX-2-deficient colorectal cancer cell line

Fri Sep 01 00:00:00 UTC 2006

Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells.

Molecular medicine (Cambridge, Mass.)

Shen C,Nathan C

PA3-750 was used in western blot to investigate the roles of Prxs in antioxidant defense in human prostate cancer cells

Fri Feb 01 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: ACR1; Alu co-repressor 1; AOEB166; B166; HBP23; Heme-binding 23 kDa protein; macrophage 23 Kd stress protein; Macrophage 23 kDa stress protein; macrophage 23kDa stress protein; macrophase stress protein 22kDa; macrophase stress protein 23 kd; Natural killer cell-enhancing factor A; natural killer-enhancing factor A; NKEF-A; OSF-3; osteoblast specific factor 3; Osteoblast-specific factor 3; PAG; Peroxiredoxin-1; proliferation-associated gene A; Proliferation-associated gene protein; Prx; Prx 1; thioredoxin dependent peroxide reductase 2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; Thioredoxin-dependent peroxiredoxin 1; Trx dependent peroxide reductase 2

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Gene Aliases: Hbp23; MSP23; NKEF-A; NKEFA; OSF-3; OSF3; PAG; PAGA; PAGB; PRDX1; PrdxI; PRX1; PRXI; TDPX2; TDX2; TPxA

UniProt ID: (Human) Q06830, (Mouse) P35700, (Rat) Q63716

Entrez Gene ID: (Human) 5052, (Mouse) 18477, (Rat) 117254

Function(s)

peroxidase activity protein binding thioredoxin peroxidase activity identical protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion antioxidant activity oxidoreductase activity heme binding protein homodimerization activity peroxiredoxin activity peroxidase

Process(es)

response to reactive oxygen species skeletal system development retina homeostasis cell proliferation removal of superoxide radicals regulation of stress-activated MAPK cascade erythrocyte homeostasis natural killer cell mediated cytotoxicity regulation of NF-kappaB import into nucleus hydrogen peroxide catabolic process cell redox homeostasis oxidation-reduction process cell-cell adhesion response to oxidative stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PRDX1 Polyclonal Antibody

PRDX1 Antibody (PA3-750) in ICC/IF

Product Details

PA3-750

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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PRDX1 Polyclonal Antibody

PRDX1 Antibody (PA3-750) in ICC/IF

Product Details

PA3-750

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

Function(s)

Process(es)

Performance Guarantee

We're here to help

Your items have has been added!