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Application
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PRPF8 Antibody (A303-921A) in IHC (P)
Detection of human PRPF8 by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-PRPF8 (Product # A303-920A) (Lot 1, left panel), (Product # A303-921A) (Lot 1, middle panel) and (Product # A303-922A) (Lot 1, right panel) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
Product Details
A303-921A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1825 and 1875 of Human Pre-mRNA-Processing Factor 8.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 220 kDa U5 snRNP-specific protein; apoptosis-regulated protein 1; apoptosis-regulated protein 2; H PRPC8; p220; Pre-mRNA-processing-splicing factor 8; precursor mRNA processing protein; PRP8 homolog; PRP8 pre-mRNA processing factor 8 homolog; spl; Splicing factor Prp8; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p
Gene Aliases: AU019467; D11Bwg0410e; DBF3/PRP8; HPRP8; PRP8; PRPC8; PRPF8; RP13; Sfprp8l; SNRNP220
UniProt ID: (Human) Q6P2Q9, (Mouse) Q99PV0
Entrez Gene ID: (Human) 10594, (Mouse) 192159
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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