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Invitrogen

PSMC3 Polyclonal Antibody

2 References

View all (39) PSMC3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Application

PSMC3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

PSMC3 Antibody (PA1-968) in ICC/IF

Immunofluorescence analysis of PSMC3 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMC3 Rabbit Polyclonal Antibody (Product # PA1-968) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

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Product Details

PA1-968

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human recombinant proteasome 19S subunit S6'.

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325951

Product Specific Information

PA1-968 detects proteasome 19S subunit S6' from human cells.

PA1-968 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 48 kDa protein representing proteasome 19S subunit S6' from HeLa cell lysate.

PA1-968 antigen is human recombinant proteasome 19S subunit S6'.

Target Information

This gene encodes a WASP interacting protein (WIP)-related protein. It has been shown that this protein has a role in the WASP-mediated organization of the actin cytoskeleton and that this protein is a potential link between the activated platelet-derived growth factor receptor and the actin polymerization machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.

Molecular & cellular proteomics : MCP

Gramolini AO,Kislinger T,Alikhani-Koopaei R,Fong V,Thompson NJ,Isserlin R,Sharma P,Oudit GY,Trivieri MG,Fagan A,Kannan A,Higgins DG,Huedig H,Hess G,Arab S,Seidman JG,Seidman CE,Frey B,Perry M,Backx PH,Liu PP,MacLennan DH,Emili A

Sat Mar 01 00:00:00 UTC 2008

Proteasomal inhibition by alpha-synuclein filaments and oligomers.

The Journal of biological chemistry

Lindersson E,Beedholm R,Højrup P,Moos T,Gai W,Hendil KB,Jensen PH

PA1-968 was used in immunoprecipitation to investigate the role of alpha-synuclein filaments and alpha-synuclein oligomers in proteasomal inhibition.

Fri Mar 26 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: 26S proteasome AAA-ATPase subunit RPT5; 26S proteasome regulatory subunit 6A; human immunodeficiency virus tat transactivator binding protein-1; MGC8487; proteasome (prosome, macropain) 26S subunit, ATPase, 3; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; rpt5; Tat-binding protein 1; TBP-1; testicular secretory protein Li 42

Gene Aliases: PSMC3; TBP1

UniProt ID: (Human) P17980

Entrez Gene ID: (Human) 5702

Function(s)

transcription coactivator activity transcription corepressor activity protein binding ATP binding ATPase activity TBP-class protein binding proteasome-activating ATPase activity protease

Process(es)

MAPK cascade protein polyubiquitination blastocyst development stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process viral process ER-associated ubiquitin-dependent protein catabolic process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability positive regulation of RNA polymerase II transcriptional preinitiation complex assembly T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway positive regulation of proteasomal protein catabolic process negative regulation of nucleic acid-templated transcription

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