Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Bethyl Laboratories
PSMD14 Polyclonal Antibody
Promotions
View available promotion(s)
Promo Code: P5906365 Don't miss out: buy 3, only pay for 2 Extended to June 27: take advantage of our 3 for 2 promotion. Whether you are restocking essentials or trying something new, now is a great time to get more for your money!. Learn more
Promo Code: RPUZZ25 Stock up on essentials to piece your discovery together Until June 27, save up to $650 and get an exclusive lab-themed hidden-object puzzle. Learn more
Application
FIGURE: 1 / 2
PSMD14 Antibody (A303-857A) in WB
Detection of human and mouse PSMD14 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (H; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMD14 antibody (Product # A303-857A) (lot A303-857A-1) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A303-857A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 260 and 310 of Human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 14.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The 26S proteasome is responsible for most intracellular proteolysis in eukaryotes. POH1 (also known as Rpn11 in yeast) subunit of the 19S complex is responsible for substrate deubiquitination during proteasomal degradation. POH1 appears to be a Zn21-dependent protease, unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases. POH1 was first identified as the human functional homolog of the S. pombe pad1 gene, which confers moderate resistance to chemotherapeutic drugs, and also to ultraviolet light when transiently overproduced in mammalian cells. Poh1-induced drug resistance is mediated through AP-1 transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 26S proteasome non-ATPase regulatory subunit 14; 26S proteasome non-ATPase subunit; 26S proteasome regulatory subunit RPN11; 26S proteasome-associated pad1 homolog; 26S proteasome-associated PAD1 homolog 1; MAD1; proteasome (prosome, macropain) 26S subunit, non-ATPase, 14; proteasome 26S subunit, non-ATPase 14; testis tissue sperm-binding protein Li 69n
Gene Aliases: 2610312C03Rik; 3200001M20Rik; AA986732; PAD1; POH1; PSMD14; rpm11; RPN11
UniProt ID: (Human) O00487, (Mouse) O35593
Entrez Gene ID: (Human) 10213, (Mouse) 59029
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support