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Invitrogen

PTCH Polyclonal Antibody

View all (42) PTCH antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Application

FIGURE: 1 / 1

PTCH Antibody (PA1-4134) in WB

Western blot detection of human PTCH1 from human fetal brain lysate using Product # PA1-4134.

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Product Details

PA1-4134

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues K(269) K I N Y Q V D S W E(279) of mouse PTCH.

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Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2174042

Product Specific Information

PA1-4134 detects Patched (PTCH) in human samples.

PA1-4134 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~158 kDa protein representing PTCH in fetal brain lysate.

The PA1-4134 immunogen is a synthetic peptide corresponding to residues K(269) K I N Y Q V D S W E(279) of mouse PTCH. This sequence is 100% conserved in human, rat, and chicken PTCH, and is expected to cross react with these samples.

Target Information

This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: etID309849.2; FLJ26746; FLJ42602; HGNC:9585; mesenchymal dysplasia; OTTHUMP00000021709; OTTHUMP00000021710; patched; Patched 1; patched homolog 1; patched homolog 2; patched protein; patched-like protein; patched1; Protein patched homolog 1; PTC; ptc-1; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH protein -3; PTCH protein -3,4,5; Ptch1 protein +12b; RP11-435O5.3

Gene Aliases: A230106A15Rik; BCNS; HPE7; mes; NBCCS; PTC; PTC1; PTCH; PTCH1; PTCH11; Ptch2

UniProt ID: (Human) Q13635, (Mouse) Q61115

Entrez Gene ID: (Human) 5727, (Chicken) 395806, (Rat) 89830, (Mouse) 19206

Function(s)

patched binding smoothened binding protein binding hedgehog receptor activity heparin binding cholesterol binding cyclin binding protein complex binding hedgehog family protein binding molecular_function zinc ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter branching involved in ureteric bud morphogenesis in utero embryonic development cell fate determination neural tube formation neural tube closure heart morphogenesis signal transduction smoothened signaling pathway regulation of mitotic cell cycle pattern specification process brain development negative regulation of cell proliferation epidermis development regulation of smoothened signaling pathway response to mechanical stimulus organ morphogenesis dorsal/ventral pattern formation epidermal cell fate specification response to chlorate positive regulation of cholesterol efflux response to organic cyclic compound protein processing spinal cord motor neuron differentiation neural tube patterning dorsal/ventral neural tube patterning neural plate axis specification embryonic limb morphogenesis mammary gland development response to estradiol response to retinoic acid regulation of protein localization limb morphogenesis hindlimb morphogenesis regulation of growth negative regulation of multicellular organism growth regulation of cell proliferation response to drug glucose homeostasis negative regulation of sequence-specific DNA binding transcription factor activity keratinocyte proliferation positive regulation of epidermal cell differentiation negative regulation of osteoblast differentiation negative regulation of smoothened signaling pathway embryonic organ development negative regulation of epithelial cell proliferation negative regulation of cell division pharyngeal system development mammary gland duct morphogenesis mammary gland epithelial cell differentiation smoothened signaling pathway involved in dorsal/ventral neural tube patterning cell differentiation involved in kidney development somite development cellular response to cholesterol renal system development cell proliferation involved in metanephros development protein targeting to plasma membrane positive regulation of transcription, DNA-templated

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