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Invitrogen

PU.1 Monoclonal Antibody (phpu13), eBioscience™

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1 Reference

View all (33) PU.1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Advanced Verification (1)

Application

PU.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

PU.1 Antibody (14-9819-82) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma, using 10 µg/mL of mouse IgG1 isotype control (left) or 10 µg/mL Anti-Human/Mouse PU-1 Purified (right) followed by biotinylated anti-mouse IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.

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PU.1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of PU.1 was observed in RAW264.7 in comparison to 3T3-L1 using Anti-PU.1 Monoclonal Antibody (phpu13), eBioscience™ (Product # 14-9819-82) in Western Blot. {RE}

Product Details

14-9819-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

phpu13

Immunogen

E. coli-derived protein, without DNA domain

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11151144

Product Specific Information

Description: The monoclonal phpu13 recognizes human and mouse PU.1, a member of the ets family of transcription factors. This 42 kDa protein was originally identified as Spi-1, a proto-oncogene, for its role in Friend virus-induced murine erythroleukemia. Expression of PU.1 plays a critical role in lymphocyte and myeloid development. Knock-out mice are neonatal lethal and lack myeloid, T and B cells. In addition erythroid development is also altered. Protein expression levels help guarantee proper development; higher levels in myeloid cells compared to lymphocytes. PU.1 controls transcription of several proteins including CD11b, CD18, Gata2, IRF4, CD20, CD10, and CD79.

Disruption of PU.1 expression may play a role in several specific leukemias; such as B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin.

Applications Reported: This phpu13 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections and immunocytochemistry.

Applications Tested: This phpu13 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58). This can be used at less than or equal to 10 µg/mL. It is recommeded that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Microglial pannexin-1 channel activation is a spinal determinant of joint pain.

Science advances

Mousseau M,Burma NE,Lee KY,Leduc-Pessah H,Kwok CHT,Reid AR,O'Brien M,Sagalajev B,Stratton JA,Patrick N,Stemkowski PL,Biernaskie J,Zamponi GW,Salo P,McDougall JJ,Prescott SA,Matyas JR,Trang T

14-9819 was used in Immunohistochemistry to describe Panx1-targeted therapy as a new mechanistic approach for alleviating joint pain.

Wed Aug 01 00:00:00 UTC 2018

Microglial pannexin-1 channel activation is a spinal determinant of joint pain.

Science advances

Mousseau M,Burma NE,Lee KY,Leduc-Pessah H,Kwok CHT,Reid AR,O'Brien M,Sagalajev B,Stratton JA,Patrick N,Stemkowski PL,Biernaskie J,Zamponi GW,Salo P,McDougall JJ,Prescott SA,Matyas JR,Trang T

14-9819 was used in Immunohistochemistry to describe Panx1-targeted therapy as a new mechanistic approach for alleviating joint pain.

Wed Aug 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: 31 kDa transforming protein; 31 kDa-transforming protein; 31kDa Transforming Protein; hCG_25181; hematopoietic transcription factor PU.1; SFFV proviral integration 1 protein; SPIA; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1; Transcription Factor spi1

Gene Aliases: Dis-1; Dis1; OF; PU.1; Sfpi-1; SFPI1; SPI-1; SPI-A; SPI1; Tcfpu1; Tfpu.1

UniProt ID: (Human) P17947, (Mouse) P17433

Entrez Gene ID: (Human) 6688, (Mouse) 20375

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA binding protein binding NFAT protein binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding sequence-specific DNA binding winged helix/forkhead transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter lymphoid progenitor cell differentiation transcription from RNA polymerase II promoter lymphocyte differentiation erythrocyte differentiation macrophage differentiation granulocyte differentiation somatic stem cell population maintenance myeloid dendritic cell differentiation histone H3 acetylation hypermethylation of CpG island negative regulation of MHC class II biosynthetic process regulation of erythrocyte differentiation negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter anatomical structure regression cellular response to ethanol negative regulation of histone H4 acetylation apoptotic process involved in patterning of blood vessels positive regulation of pri-miRNA transcription from RNA polymerase II promoter vasculature development myeloid leukocyte differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PU.1 Monoclonal Antibody (phpu13), eBioscience™

PU.1 Antibody (14-9819-82) in IHC (P)

Product Details

14-9819-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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