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Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, PLIN1 A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kD, respectively. The N-terminus, however, remains unchanged. The antibody reacts specifically with all PLIN1 variants located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, sebaceous gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. It also is a useful pathological marker for steatogenesis e.g. in liver. It does not cross-react with adipophilin (ADRP, also named PLIN2) or TIP47 (also named PLIN3) proteins (or additional members of the PLIN/PAT-family, e.g. MLDP or OXPAT/PAT-1, also named PLIN5 or LSDP5). Tested reactivity on cultured cell lines: Several human carcinoma cell lines; 3T3-L1 adipocytes.
Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
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Protein Aliases: Lipid droplet-associated protein; perilipin; Perilipin A; perilipin B; Perilipin-1
Gene Aliases: 6030432J05Rik; FPLD4; PERI; PLIN; PLIN1
UniProt ID: (Human) O60240, (Mouse) Q8CGN5
Entrez Gene ID: (Human) 5346, (Mouse) 103968
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