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            • Primary Antibodies ›
            • 14-3-3 zeta Antibodies

            Invitrogen

            Phospho-14-3-3 zeta (Ser58) Polyclonal Antibody

            View all (29) 14-3-3 zeta antibodies
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            Cite Phospho-14-3-3 zeta (Ser58) Polyclonal Antibody

            • Antibody Testing Data (3)
            Application
            Phospho-14-3-3 zeta (Ser58) Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            Phospho-14-3-3 zeta (Ser58) Antibody (PA5-104934) in ICC/IF

            Immunofluorescent analysis of Phospho-14-3-3 zeta (Ser58) in HeLa cells treated with PMA (125 ng/mL). Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Phospho-14-3-3 zeta (Ser58) polyclonal antibody (Product # PA5-104934) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of... View More
            View Product

            Phospho-14-3-3 zeta (Ser58) Antibody in Immunocytochemistry (ICC/IF)
            Phospho-14-3-3 zeta (Ser58) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Phospho-14-3-3 zeta (Ser58) Antibody in Western Blot (WB)

            Product Details

            PA5-104934

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:2,000
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:200
            -

            Immunocytochemistry (ICC/IF)

            1:100-1:500
            -
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            A synthesized peptide derived from human YWHAZ(Accession P63104), corresponding to amino acid residues around phosphorylated Ser58.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            sequential chromatography

            Storage buffer

            PBS, pH 7.4, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2816407

            Product Specific Information

            Antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Serine 58.

            Target Information

            At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: 14-3-3 delta; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; 1433Z; 143Z; epididymis luminal protein 4; epididymis secretory protein Li 3; epididymis secretory protein Li 93; KCIP-1; Mitochondrial import stimulation factor S1 subunit; phospholipase A2; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; SEZ-2; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide

            View more View less

            Gene Aliases: 1110013I11Rik; 14-3-3-zeta; 14-3-3z; 14-3-3zeta; AI596267; AL022924; AU020854; HEL-S-3; HEL-S-93; HEL4; KCIP-1; Msfs1; YWHAD; YWHAZ

            View more View less

            UniProt ID: (Human) P63104, (Mouse) Q3TSF1, (Rat) P63102

            View more View less

            Entrez Gene ID: (Human) 7534, (Mouse) 22631, (Rat) 25578

            View more View less

            Function(s)

            protein binding transcription factor binding protein kinase binding protein domain specific binding ubiquitin protein ligase binding identical protein binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion protein complex binding scaffold/adaptor protein

            Process(es)

            protein targeting signal transduction platelet activation negative regulation of apoptotic process regulation of mRNA stability establishment of Golgi localization membrane organization Golgi reassembly cell-cell adhesion positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway histamine secretion by mast cell protein targeting to mitochondrion regulation of cell death response to drug
            It has to be done as per old AB suggested Products section.
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