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            • Primary Antibodies ›
            • ACK1 Antibodies

            Invitrogen

            Phospho-ACK1 (Tyr284) Polyclonal Antibody

            View all (24) ACK1 antibodies
            Datasheet
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            Catalog # PA5-105624
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            Price (USD)
            477.00
            100 µL
             
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            Cite Phospho-ACK1 (Tyr284) Polyclonal Antibody

            • Antibody Testing Data (4)
            Application
            Phospho-ACK1 (Tyr284) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Group 53 Created with Sketch.

            FIGURE: 1 / 4

            Phospho-ACK1 (Tyr284) Antibody (PA5-105624) in IHC (P)

            Immunohistochemistry analysis of paraffin-embedded Phospho-ACK1 (Tyr284) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-ACK1 (Tyr284) polyclonal antibody (Product # PA5-105624) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
            View Product

            Phospho-ACK1 (Tyr284) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Phospho-ACK1 (Tyr284) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Phospho-ACK1 (Tyr284) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Phospho-ACK1 (Tyr284) Antibody in Western Blot (WB)

            Product Details

            PA5-105624

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:2,000
            -

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:200
            -
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            A synthesized peptide derived from human TNK2(Accession Q07912), corresponding to amino acid residues around phosphorylated Tyr284.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            sequential chromatography

            Storage buffer

            PBS, pH 7.4, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2817052

            Product Specific Information

            Antibody detects endogenous levels of ACK1 only when phosphorylated at Tyr284.

            Target Information

            TNK2 (ACK) (activated Cdc42-associated kinase) is a non-receptor tyrosine kinase that signals downstream of Cdc42. TNK2 is known to be involved in tumorigenesis and metastasis, particularly in prostate cancer. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: ACK-1; Activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; Cdc42 GTPase-inhibiting protein; Non-receptor protein tyrosine kinase Ack; P21cdc42Hs kinase; tnk2 {ECO:0000250|UniProtKB:Q07912}; Tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2

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            Gene Aliases: ACK; ACK-1; ACK1; Cdgip; p21cdc42Hs; Pyk1; TNK2

            View more View less

            UniProt ID: (Human) Q07912, (Mouse) O54967, (Rat) Q5U2X5

            View more View less

            Entrez Gene ID: (Human) 10188, (Mouse) 51789, (Rat) 303882

            View more View less

            Function(s)

            protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity GTPase inhibitor activity epidermal growth factor receptor binding protein binding ATP binding identical protein binding metal ion binding WW domain binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups hormone receptor binding

            Process(es)

            protein phosphorylation endocytosis signal transduction transmembrane receptor protein tyrosine kinase signaling pathway phosphorylation cell migration peptidyl-tyrosine phosphorylation cell differentiation peptidyl-tyrosine autophosphorylation regulation of cell proliferation innate immune response positive regulation of peptidyl-tyrosine phosphorylation regulation of clathrin-mediated endocytosis cell surface receptor signaling pathway small GTPase mediated signal transduction negative regulation of GTPase activity
            It has to be done as per old AB suggested Products section.
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