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AbboMax

Phospho-AXL/UFO (Tyr857) Polyclonal Antibody

View all (67) AXL antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Application

Phospho-AXL/UFO (Tyr857) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

Phospho-AXL/UFO (Tyr857) Antibody (630-990) in IHC (P)

Immunohistochemistry: Human breast carcinoma (FFPE) stained with Rabbit anti-AXL (pY857) (Cat# 630-990) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Phospho-AXL/UFO (Tyr857) Antibody in Western Blot (WB)

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Product Details

630-990

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide surrounding to the epitope -EVHPAGR-Y-VL- with a phosphorylation site at Tyr812 of human AXL protein

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

25mM tris with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Hela cells.

Cellular location: Membrane.

Target Information

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Adhesion-related kinase; AXL oncogene; AXL transforming sequence/gene; AZF1; oncogene AXL; sAXL; soluble AXL; Tyrosine-protein kinase receptor UFO; ufo oncogene homolog

Gene Aliases: AI323647; ARK; AXL; JTK11; Tyro7; UFO

UniProt ID: (Human) P30530, (Mouse) Q00993

Entrez Gene ID: (Human) 558, (Mouse) 26362, (Rat) 308444

Function(s)

virus receptor activity phosphatidylserine binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding protein heterodimerization activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups myosin heavy chain binding phosphatidylinositol 3-kinase binding growth factor binding transmembrane signal receptor

Process(es)

neuron migration natural killer cell differentiation positive regulation of cytokine-mediated signaling pathway blood vessel remodeling phagocytosis inflammatory response signal transduction spermatogenesis peptidyl-tyrosine phosphorylation forebrain cell migration platelet activation cellular response to extracellular stimulus negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of natural killer cell differentiation secretion by cell erythrocyte homeostasis substrate adhesion-dependent cell spreading cellular response to interferon-alpha ovulation cycle apoptotic cell clearance protein kinase B signaling negative regulation of neuron apoptotic process innate immune response viral entry into host cell vascular endothelial growth factor receptor signaling pathway cell maturation positive regulation of pinocytosis negative regulation of lymphocyte activation positive regulation of protein kinase B signaling vagina development cellular response to lipopolysaccharide dendritic cell differentiation negative regulation of dendritic cell apoptotic process immune system process protein phosphorylation enzyme linked receptor protein signaling pathway phosphorylation cell differentiation negative regulation of apoptotic process cellular response to hydrogen peroxide cell proliferation cell migration organ regeneration tissue regeneration tissue remodeling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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