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Invitrogen
Phospho-BLNK (Tyr96) Polyclonal Antibody
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Application
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Phospho-BLNK (Tyr96) Antibody (PA5-105018) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-BLNK (Tyr96) in human lymph node tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-BLNK (Tyr96) polyclonal antibody (Product # PA5-105018) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105018
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BLNK(Accession Q8WV28), corresponding to amino acid residues around phosphorylated Tyr96.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816491
Product Specific Information
Antibody detects endogenous levels of BLNK only when phosphorylated at Tyrosine 96.
Target Information
B cell linker protein (Blnk) is a protein encoded by the Blnk gene located on chromosome 10 in humans. It is also known as SLP 65 (Src homology 2 domain-containing leukocyte protein of 65 kDa). It derives its name from being an adaptor protein in the B cell receptor signaling cascade with several supporting studies showing improper development of B cells in the absence of Blnk. Blnk undergoes tyrosine phosphorylation by Syk, a B cell receptor activated kinase, which provides a platform for interaction of signaling intermediaries such as the Vav GEF and Grb2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell adaptor containing SH2 domain; B cell linker protein; B-cell activation; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker protein; Cytoplasmic adapter protein; Lymphocyte antigen 57; MGC111051; SLP-65; Src homology 2 domain-containing leukocyte protein of 65 kDa; Src homology [SH2] domain-containing leukocyte protein of 65 kD
Gene Aliases: AGM4; BASH; Bca; BLNK; BLNK-S; Ly-57; LY57; Lyw-57; SLP-65; SLP65
UniProt ID: (Human) Q8WV28, (Mouse) Q9QUN3
Entrez Gene ID: (Human) 29760, (Mouse) 17060
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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