Invitrogen

Phospho-BRG1 (Ser610) Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-105089

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SMARCA4(Accession P51532), corresponding to amino acid residues around phosphorylated Ser610.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816562

Product Specific Information

Antibody detects endogenous levels of BRG1 only when phosphorylated at Ser610.

Target Information

Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent helicase SMARCA4; BAF190A; brahma protein-like 1; BRG1-associated factor 190A; BRM/SWI2-related gene 1; FLJ39786; global transcription activator homologous sequence; homeotic gene regulator; Mitotic growth and transcription activator; nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; SNF2-beta; SNF2-like 4; sucrose nonfermenting-like 4; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; Transcription activator BRG1

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Gene Aliases: b2b508.1Clo; b2b692Clo; BAF190; BAF190A; brg-1; BRG1; CSS4; HP1-BP72; hSNF2b; MRD16; RTPS2; SMARCA4; SNF2; SNF2B; SNF2beta; SNF2L4; SNF2LB; SW1/SNF; SWI2

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UniProt ID: (Human) P51532, (Mouse) Q3TKT4

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Entrez Gene ID: (Human) 6597, (Mouse) 20586, (Rat) 171379

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription coactivator activity RNA polymerase I CORE element sequence-specific DNA binding p53 binding transcription coactivator activity transcription corepressor activity helicase activity protein binding ATP binding DNA-dependent ATPase activity transcription factor binding Tat protein binding nucleosomal DNA binding protein N-terminus binding androgen receptor binding DNA polymerase binding lysine-acetylated histone binding nucleotide binding RNA polymerase II regulatory region sequence-specific DNA binding DNA binding chromatin binding hydrolase activity hydrolase activity, acting on acid anhydrides hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides ATPase activity protein complex binding histone binding DNA helicase
Process(es)
negative regulation of transcription from RNA polymerase II promoter neural retina development nucleosome disassembly chromatin remodeling transcription, DNA-templated regulation of transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter during mitosis spermatid development nervous system development covalent chromatin modification positive regulation of Wnt signaling pathway negative regulation of cell growth ATP-dependent chromatin remodeling positive regulation by host of viral transcription negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of androgen receptor signaling pathway positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of glucose mediated signaling pathway positive regulation of pri-miRNA transcription from RNA polymerase II promoter beta-catenin-TCF complex assembly negative regulation of G1/S transition of mitotic cell cycle cell morphogenesis blood vessel development vasculogenesis in utero embryonic development blastocyst growth blastocyst hatching liver development outflow tract morphogenesis nucleosome assembly methylation-dependent chromatin silencing regulation of transcription, DNA-templated transcription from RNA polymerase II promoter glial cell fate determination heart development positive regulation of cell proliferation DNA methylation on cytosine within a CG sequence chromatin modification stem cell population maintenance neurogenesis extracellular matrix organization keratinocyte differentiation regulation of cell migration forebrain development hindbrain development embryonic hindlimb morphogenesis aortic smooth muscle cell differentiation negative regulation of apoptotic process positive regulation of DNA binding histone H3 acetylation positive regulation of cell differentiation embryonic organ morphogenesis epidermis morphogenesis definitive erythrocyte differentiation heart trabecula formation pharyngeal arch artery morphogenesis lens fiber cell development
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