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Invitrogen

Phospho-CDK2 (Thr160) Polyclonal Antibody

View all (72) CDK2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Application

Phospho-CDK2 (Thr160) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

Phospho-CDK2 (Thr160) Antibody (PA5-117192) in IHC (P)

Immunohistochemistry analysis of Phospho-CDK2 (Thr160) in paraffin-embedded human breast carcinoma. Samples were incubated with Phospho-CDK2 (Thr160) Polyclonal antibody (Product # PA5-117192). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Phospho-CDK2 (Thr160) Antibody in Western Blot (WB)

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Product Details

PA5-117192

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around T160 of human CDK2 (NP_0017892?/NP_0012491)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.55 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2901822

Product Specific Information

Positive Samples: HeLa

Immunogen sequence: TYTHE

Target Information

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; CDK1; Cell division protein kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2; p34 protein kinase

Gene Aliases: A630093N05Rik; CDK2; CDKN2; p33(CDK2)

UniProt ID: (Human) P24941, (Mouse) P97377

Entrez Gene ID: (Human) 1017, (Rat) 362817, (Mouse) 12566

Function(s)

protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding kinase activity cyclin binding protein complex binding histone kinase activity metal ion binding protein kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle lens development in camera-type eye DNA replication DNA repair potassium ion transport DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division centriole replication meiotic nuclear division Ras protein signal transduction positive regulation of cell proliferation response to toxic substance histone phosphorylation peptidyl-serine phosphorylation organ regeneration mitotic G1 DNA damage checkpoint positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus response to drug response to ethanol positive regulation of transcription, DNA-templated response to cadmium ion centrosome duplication cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle response to cAMP response to electrical stimulus regulation of gene silencing cellular response to nitric oxide regulation of signal transduction by p53 class mediator protein phosphorylation meiotic cell cycle regulation of cell cycle cellular response to DNA damage stimulus cell cycle phosphorylation

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