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            • Primary Antibodies ›
            • ERK1/2 Antibodies

            AbboMax

            Phospho-ERK1/2 (P44-MAPK) (Thr202, Tyr204) Polyclonal Antibody

            查看其他61个ERK1/2抗体
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            Cite Phospho-ERK1/2 (P44-MAPK) (Thr202, Tyr204) Polyclonal Antibody

            • 抗体检测数据 (1)
            Application
            Phospho-ERK1/2 (P44-MAPK) (Thr202, Tyr204) Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

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            Phospho-ERK1/2 (P44-MAPK) (Thr202, Tyr204) Antibody (602-310) in WB

            Western Blot: The whole cell lysate derived from UV treated 3T3 was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti – ERK1/2 (pT202/pY204) (Cat#602-310) antibody at 1:500. A dual immunoreactive band around ~42/44 kDa is observed.
            查看产品

            Phospho-ERK1/2 (P44-MAPK) (Thr202, Tyr204) Antibody in Western Blot (WB)

            产品信息

            602-310

            应用
            建议稀释比
            已发表文章

            免疫印迹 (WB)

            0.1-1 µg/mL
            -

            免疫组化(石蜡) (IHC (P))

            2-10 µg/mL
            -
            产品规格

            种属反应

            Chicken, Human, Mouse, Rat

            宿主/亚型

            Rabbit / IgG

            分类

            Polyclonal

            类型

            Antibody

            抗原

            A synthetic peptide derived from epitope -LTEYV- with the dual phosphorylation sites Thr202 and Tyr204 of ERK1/2 protein
            查看免疫原

            偶联物

            Unconjugated

            形式

            Liquid

            浓度

            0.5 mg/mL

            纯化类型

            Antigen affinity chromatography

            保存液

            25mM tris with proprietary stabilizer

            内含物

            0.01% sodium azide

            保存条件

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            运输条件

            Ambient (domestic); Wet ice (international)

            产品详细信息

            Positive control: Kidney and spleen lysates

            Cellular location: Nucleus.

            靶标信息

            ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

            仅用于科研。不用于诊断过程。未经明确授权不得转售。

            篇参考文献 (0)

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            引用该产品

            生物信息学

            蛋白别名: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein tyrosine kinase ERK2

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            基因别名: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

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            UniProt ID: (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844

            查看更多 收起

            Entrez Gene ID: (Human) 5594, (Human) 5595, (Chicken) 373953, (Mouse) 26413, (Rat) 116590, (Rat) 50689, (Mouse) 26417

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            功能

            phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase

            参与通路

            MAPK cascade activation of MAPK activity MAPK import into nucleus transcription, DNA-templated protein phosphorylation apoptotic process chemotaxis response to stress cellular response to DNA damage stimulus cell cycle signal transduction chemical synaptic transmission axon guidance positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway response to toxic substance positive regulation of peptidyl-threonine phosphorylation regulation of phosphatidylinositol 3-kinase signaling viral process peptidyl-serine phosphorylation peptidyl-threonine phosphorylation sensory perception of pain cytosine metabolic process platelet activation positive regulation of cell migration thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB signaling pathway outer ear morphogenesis response to exogenous dsRNA response to estrogen negative regulation of cell differentiation positive regulation of translation positive regulation of transcription, DNA-templated thymus development T cell receptor signaling pathway B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation long-term synaptic potentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade response to epidermal growth factor caveolin-mediated endocytosis regulation of Golgi inheritance cellular response to granulocyte macrophage colony-stimulating factor stimulus regulation of cellular response to heat positive regulation of telomere capping regulation of early endosome to late endosome transport positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly DNA damage induced protein phosphorylation phosphorylation arachidonic acid metabolic process BMP signaling pathway positive regulation of cyclase activity positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation positive regulation of transcription from RNA polymerase II promoter cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus positive regulation of xenophagy negative regulation of apolipoprotein binding organ morphogenesis response to lipopolysaccharide intracellular signal transduction cellular response to organic substance
            It has to be done as per old AB suggested Products section.
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