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Invitrogen
Phospho-ERK3 (Ser189) Polyclonal Antibody
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Application
FIGURE: 1 / 1
Phospho-ERK3 (Ser189) Antibody (PA5-97343) in WB
Western blot analysis of Phospho-ERK3 (Ser189) in extracts of 293 cells. Samples were incubated with Phospho-ERK3 (Ser189) Polyclonal antibody (Product # PA5-97343) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. 293 cells were treated by PMA/TPA (200 nM) for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg per lane. B... View More
Product Details
PA5-97343
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S189 of human ERK3 (NP_0027391)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.86 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809145
Product Specific Information
Positive Samples: 293; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: HLSEG
Target Information
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. Tissue specificity: Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686F03189; ERK-3; Erk-3 related; Extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; MAP kinase 6; MAP kinase isoform p97; MAPK 6; mitogen activated protein kinase 4; mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; p55-MAPK; p97-MAPK; protein kinase, mitogen activated kinase 4; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6
Gene Aliases: 2610021I23Rik; D130053K17Rik; ERK3; HsT17250; Mapk4; MAPK6; Mapk63; p97MAPK; Prkm4; PRKM6
UniProt ID: (Human) Q16659, (Mouse) Q61532, (Rat) P27704
Entrez Gene ID: (Human) 5597, (Mouse) 50772, (Rat) 58840
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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