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Invitrogen

Phospho-IKK alpha (Ser176, Ser180) Polyclonal Antibody

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1 Published Figure

3 References

View all (37) IKK alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

Phospho-IKK alpha (Ser176, Ser180) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Phospho-IKK alpha (Ser176, Ser180) Antibody (44-714) in ICC/IF

Immunofluorescence analysis of Phospho-IKK alpha (Ser176, Ser180) was done on 70% confluent log phase HeLa cells. The cells were treated for 20 minutes with 50 ng/mL of TNF-Alpha and fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-IKK alpha (Ser176, Ser180) Rabbit... View More

Phospho-IKK alpha (Ser176, Ser180) Antibody in Western Blot (WB)

Phospho-IKK alpha (Ser176, Ser180) Antibody in ChIP Assay (ChIP)

Phospho-IKK alpha (Ser176, Ser180) Antibody in Immunohistochemistry (IHC)

Phospho-IKK alpha (Ser176, Ser180) Antibody

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Product Details

44-714

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

ChIP assay (ChIP)

10 µL

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IKK-alpha that contains serine 176 and serine 180.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533730

Target Information

CHUK (IKK alpha) is a serine/threonine kinase involved in signal transduction and cellular communication. It belongs to the cytokine activated protein complex that is an inhibitor of the NF-kappa B complex. IKK alpha along with IKK beta and a regulatory protein NEMO (IKK gamma) phosphorylates IkB proteins which targets the later for ubiquitin mediated degradation. It is involved with several cellular processes with extensive studies on its role in inflammation and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sex Dependent Disparities in the Central Innate Immune Response after Moderate Spinal Cord Contusion in Rat.

Cells

Ghosh M,Lee J,Burke AN,Strong TA,Sagen J,Pearse DD

Published figure using Phospho-IKK alpha (Ser176, Ser180) polyclonal antibody (Product # 44-714) in Immunohistochemistry

Sat Apr 06 00:00:00 UTC 2024

Sesamol Attenuates Renal Inflammation and Arrests Reactive-Oxygen-Species-Mediated IL-1β Secretion via the HO-1-Induced Inhibition of the IKKα/NFκB Pathway In Vivo and In Vitro.

Antioxidants (Basel, Switzerland)

Tseng KF,Tsai PH,Wang JS,Chen FY,Shen MY

44-714 was used in Western Blot to explore whether Sesamol mitigates macrophage-mediated renal inflammation and its underlying mechanisms.

Wed Dec 14 00:00:00 UTC 2022

Autophagic feedback-mediated degradation of IKKα requires CHK1- and p300/CBP-dependent acetylation of p53.

Journal of cell science

Xu X,Zhang C,Xu H,Wu L,Hu M,Song L

44-714 was used in Western Blot to conclude that both IKKα- and CHK1-dependent p53 phosphorylation and acetylation contribute to mediating selective autophagy feedback degradation of IKKα during the arsenite-induced proapoptotic responses.

Mon Nov 16 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; FLJ40509; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; ikappaB kinase; IkB kinase alpha subunit; IKBKB; IKK-1; IKK-a kinase; IKK-B; IKK-beta; IKK2; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit alpha; kinase beta; MGC131801; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; TCF-16; Transcription factor 16

Gene Aliases: CHUK; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16

UniProt ID: (Human) O15111

Entrez Gene ID: (Human) 1147

Function(s)

protein kinase activity protein binding ATP binding IkappaB kinase activity protein homodimerization activity protein heterodimerization activity scaffold protein binding non-receptor serine/threonine protein kinase protein modifying enzyme

Process(es)

stimulatory C-type lectin receptor signaling pathway skeletal muscle contraction protein phosphorylation inflammatory response immune response I-kappaB kinase/NF-kappaB signaling I-kappaB phosphorylation Rho protein signal transduction response to virus response to toxic substance anatomical structure morphogenesis response to acetate regulation of tumor necrosis factor-mediated signaling pathway response to lipopolysaccharide response to hydroperoxide TRIF-dependent toll-like receptor signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway response to drug positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid innate immune response positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway cellular response to tumor necrosis factor

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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