Invitrogen

Phospho-IRS1 (Ser636) Polyclonal Antibody

View all (71) IRS1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-104876

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human IRS1(Accession P35568), corresponding to amino acid residues around phosphorylated Ser636.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816349

Product Specific Information

Antibody detects endogenous levels of IRS1 only when phosphorylated at Serine 636.

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Insulin receptor substrate 1; IRS-1; pp185

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Gene Aliases: G972R; HIRS-1; IRS-1; IRS1; IRS1IRM

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UniProt ID: (Human) P35568, (Rat) P35570, (Mouse) P35569

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Entrez Gene ID: (Human) 3667, (Rat) 25467, (Mouse) 16367

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Function(s)
signal transducer activity transmembrane receptor protein tyrosine kinase adaptor activity protein kinase C binding Ras guanyl-nucleotide exchange factor activity insulin receptor binding insulin-like growth factor receptor binding protein binding 1-phosphatidylinositol-3-kinase activity SH2 domain binding phosphatidylinositol 3-kinase binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein kinase binding protein domain specific binding
Process(es)
MAPK cascade signal transduction positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of glucose metabolic process phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling positive regulation of fatty acid beta-oxidation response to insulin cellular response to insulin stimulus phosphatidylinositol-3-phosphate biosynthetic process glucose homeostasis response to peptide hormone positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity positive regulation of glycogen biosynthetic process positive regulation of glucose import negative regulation of insulin receptor signaling pathway positive regulation of insulin receptor signaling pathway negative regulation of insulin secretion phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of glucose import in response to insulin stimulus positive regulation of mesenchymal cell proliferation regulation of gene expression lipid catabolic process insulin secretion positive regulation of cell migration mammary gland development response to caffeine protein localization to nucleus positive regulation of phosphorylation protein kinase B signaling protein heterooligomerization positive regulation of glucagon secretion negative regulation of somatostatin secretion
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