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Invitrogen
Phospho-LATS1 (Ser909) Polyclonal Antibody
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Application
FIGURE: 1 / 2
Phospho-LATS1 (Ser909) Antibody (PA5-105443) in ICC/IF
Immunocytochemistry analysis of Phospho-LATS1 (Ser909) in Hela cells (20 min of 30 µM Forskolin treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-LATS1 (Ser909) polyclonal antibody (Product # PA5-105443) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated g... View More
Product Details
PA5-105443
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LATS1(Accession O95835), corresponding to amino acid residues around phosphorylated Ser909.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816871
Product Specific Information
Antibody detects endogenous levels of LATS1 only when phosphorylated at Ser909.
Target Information
LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis which is phosphorylated in a cell-cycle dependent manner. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity which indicating a role as a negative regulator of CDC2/cyclin A and the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. LATS1 act as a tumor suppressor and play an important role in the development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: h-warts; Large tumor suppressor homolog 1; large tumor supressor, homolog 1; LATS (large tumor suppressor, Drosophila) homolog 1; LATS, large tumor suppressor, homolog 1; LATS1; Serine/threonine-protein kinase LATS1; WARTS protein kinase
Gene Aliases: AW208599; LATS1; WARTS; wts
UniProt ID: (Human) O95835, (Mouse) Q8BYR2
Entrez Gene ID: (Human) 9113, (Mouse) 16798
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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