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Invitrogen
Phospho-MEK1/MEK2 (Ser217, Ser221) Recombinant Rabbit Monoclonal Antibody (MEK12S217S221-H2), FITC
FIGURE: 1 / 1
Phospho-MEK1/MEK2 (Ser217, Ser221) Antibody (MA5-37098) in Flow
Flow cytometry of Phospho-MEK1/2 (Ser217, Ser221) using HeLa cells. Samples were incubated in monoclonal Phospho-MEK1/2 (Ser217, Ser221) antibody (Product # MA5-37098). Images correspond with the following conditions: treated with imatinib and unstained as negative control (blue), treated with imatinib (red), treated with pervanadate (green).
Product Details
MA5-37098
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
MEK12S217S221-H2
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser217/221 of human phospho MEK1/2.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
PBS with 0.20% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897032
Target Information
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In brain, this pathway also plays a key role in synaptic (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; EC 2.7.12.2; ERK activator kinase 1; ERK activator kinase 2; kinase MEK1; MAP kinase kinase 1; MAP kinase kinase 2; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MEK 1; MEK 2; MEK-1; MEK-2; mitogen-activated protein kinase kinase 2, p45; MP2K1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Aliases: CFC3; CFC4; MAP2K1; MAP2K2; MAPKK1; MAPKK2; MEK1; MEK2; MKK1; MKK2; PRKMK1; PRKMK2
UniProt ID: (Human) Q02750, (Human) P36507
Entrez Gene ID: (Human) 5604, (Human) 5605
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
receptor signaling protein tyrosine phosphatase activity
protein binding
ATP binding
protein C-terminus binding
protein kinase binding
protein serine/threonine kinase activator activity
protein N-terminus binding
PDZ domain binding
protein complex scaffold
metal ion binding
scaffold protein binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
movement of cell or subcellular component
chemotaxis
cell cycle arrest
signal transduction
heart development
negative regulation of cell proliferation
positive regulation of gene expression
negative regulation of gene expression
peptidyl-tyrosine phosphorylation
cerebellar cortex formation
neuron differentiation
keratinocyte differentiation
thyroid gland development
regulation of stress-activated MAPK cascade
proteolysis in other organism
positive regulation of transcription, DNA-templated
thymus development
regulation of axon regeneration
cell motility
positive regulation of axonogenesis
Bergmann glial cell differentiation
face development
trachea formation
epithelial cell proliferation involved in lung morphogenesis
placenta blood vessel development
labyrinthine layer development
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein serine/threonine kinase activity
regulation of Golgi inheritance
cellular senescence
mitophagy in response to mitochondrial depolarization
positive regulation of production of miRNAs involved in gene silencing by miRNA
regulation of early endosome to late endosome transport
peptidyl-serine autophosphorylation
positive regulation of cell motility
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