Search Thermo Fisher Scientific
- Contact Us
- Quick Order
-
Don't have an account ? Create Account
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phospho-MSK2 (Ser360) Polyclonal Antibody
Application
FIGURE: 1 / 5
Phospho-MSK2 (Ser360) Antibody (PA5-105431) in ICC/IF
Immunocytochemistry analysis of Phospho-MSK2 (Ser360) in H2O2 treated HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-MSK2 (Ser360) polyclonal antibody (Product # PA5-105431) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (... View More
Product Details
PA5-105431
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RPS6KA4(Accession O75676), corresponding to amino acid residues around phosphorylated Ser360.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816859
Product Specific Information
Antibody detects endogenous levels of MSK2 only when phosphorylated at Ser360.
Target Information
RPS6KA4 (MSK2) is a serine/threonine protein kinase that is essential for the regulation of various cellular processes. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. RPS6KA4 contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 90 kDa ribosomal protein S6 kinase 4; coiled-coil domain containing 88B; EC 2.7.11.1; kinase MSK2; KS6A4; mitogen- and stress-activated protein kinase 2; mitogen- and stress-activated protein kinase-2; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; ribosomal protein S6 kinase, 90kD, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RLSK; RSK-like protein kinase; RSKB; S6K-alpha-4
Gene Aliases: 1110069D02Rik; 90kDa; AI848992; Ccdc88b; mMSK2; MSK2; RPS6KA4; RSK-B; S6K-alpha-4
UniProt ID: (Human) O75676, (Mouse) Q9Z2B9
Entrez Gene ID: (Human) 8986, (Mouse) 56613, (Rat) 361715
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support