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Phospho-NFAT5 (Ser134) Antibody (PA5-105437) in ICC/IF
Immunocytochemistry analysis of Phospho-NFAT5 (Ser134) in H2O2 treated HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-NFAT5 (Ser134) polyclonal antibody (Product # PA5-105437) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L)... View More
Product Details
PA5-105437
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NFAT5(Accession O94916), corresponding to amino acid residues around phosphorylated Ser134.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816865
Product Specific Information
Antibody detects endogenous levels of NFAT5 only when phosphorylated at Ser134.
Target Information
The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glutamine rich protein H65; NF-AT5; NFAT-like protein 1; Nuclear factor of activated T-cells 5; osmotic response element-binding protein; Rel domain-containing transcription factor NFAT5; T-cell transcription factor NFAT5; TonE-binding protein; tonicity-responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein
Gene Aliases: AI225870; B130038B15Rik; CAG-8; CAG80; KIAA0827; mKIAA0827; NF-AT5; NFAT5; NFATL1; NFATZ; OREBP; TONEBP
UniProt ID: (Human) O94916, (Mouse) Q9WV30
Entrez Gene ID: (Human) 10725, (Mouse) 54446
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
DNA binding
Rel homology transcription factor
cytokine production
transcription from RNA polymerase II promoter
response to osmotic stress
signal transduction
excretion
positive regulation of transcription from RNA polymerase II promoter
regulation of calcineurin-NFAT signaling cascade
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of gene expression
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