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Invitrogen
Phospho-PAK4/6/7 (Ser474) Polyclonal Antibody
Product Details
PA5-64495
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PAK4/5/6 (Phospho-Ser474)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2663447
Product Specific Information
Phospho-PAK4/PAK5/PAK6 (Ser474) Polyclonal Antibody detects endogenous levels of PAK4/PAK5/PAK6 only when phosphorylated at Ser474.
Target Information
PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction. PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: p21 (CDKN1A)-activated kinase 4; p21 (CDKN1A)-activated kinase 6; p21 (CDKN1A)-activated kinase 7; p21 (RAC1) activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A)-activated kinase 4; p21(CDKN1A)-activated kinase 6; p21(CDKN1A)-activated kinase 7; p21-activated kinase 4; p21-activated kinase 5; p21-activated kinase 6; p21-activated kinase 7; p21CDKN1A-activated kinase 7; PAK-4; PAK-5; PAK-6; PAK-7; protein kinase PAK5; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; Serine/threonine-protein kinase PAK 4; Serine/threonine-protein kinase PAK 5; Serine/threonine-protein kinase PAK 6; serine/threonine-protein kinase PAK 7; serine/threonine-protein kinase PAK7
Gene Aliases: 2900083L08Rik; 4732456M09; 5730488L07Rik; 6430627N20; AW555722; KIAA1142; KIAA1264; mKIAA1142; PAK-5; PAK-7; PAK4; PAK5; PAK6; PAK7
UniProt ID: (Human) O96013, (Human) Q9P286, (Human) Q9NQU5, (Mouse) Q8BTW9, (Mouse) Q3ULB5, (Rat) D4A280, (Mouse) Q8C015
Entrez Gene ID: (Human) 10298, (Human) 57144, (Human) 56924, (Mouse) 70584, (Rat) 292756, (Mouse) 214230, (Rat) 296078, (Rat) 311450, (Mouse) 241656
protein kinase activity
receptor signaling protein serine/threonine kinase activity
protein binding
ATP binding
cadherin binding involved in cell-cell adhesion
protein serine/threonine kinase activity
Rac GTPase binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
mitotic cell cycle
apoptotic process
movement of cell or subcellular component
cytoskeleton organization
signal transduction
cell proliferation
cell growth
cell migration
signal transduction by protein phosphorylation
dendritic spine development
cellular response to organic cyclic compound
cell-cell adhesion
learning
memory
locomotory behavior
negative regulation of extrinsic apoptotic signaling pathway
regulation of transcription, DNA-templated
protein phosphorylation
Rho protein signal transduction
regulation of mitotic cell cycle
actin cytoskeleton organization
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
regulation of MAPK cascade
intracellular signal transduction
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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