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Invitrogen
Phospho-PDE6 gamma (Thr22) Polyclonal Antibody
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Application
FIGURE: 1 / 1
Phospho-PDE6 gamma (Thr22) Antibody (PA5-106023) in WB
Western blot analysis of Phospho-PDE6 gamma (Thr22) in EGF treated 293 cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-PDE6 gamma (Thr22) polyclonal antibody (Product # PA5-106023).
Product Details
PA5-106023
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PDE6G(Accession P18545), corresponding to amino acid residues around phosphorylated Thr22.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2817422
Product Specific Information
Antibody detects endogenous levels of PDE6G only when phosphorylated at Thr22.
Target Information
The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha' (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha'/alpha' homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GMP-PDE gamma; phosphodiesterase 6G, cGMP-specific, rod, gamma; Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma; rod cG-PDE G
Gene Aliases: p3AT; PDE6G; PDEG; RP57
UniProt ID: (Human) P18545, (Mouse) P09174
Entrez Gene ID: (Human) 5148, (Rat) 688297, (Mouse) 18588
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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