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            • Primary Antibodies ›
            • PFKP Antibodies

            Invitrogen

            Phospho-PFKP (Tyr52) Polyclonal Antibody

            View all (45) PFKP antibodies
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            Datasheet
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            Cite Phospho-PFKP (Tyr52) Polyclonal Antibody

            • Antibody Testing Data (1)
            Application
            Phospho-PFKP (Tyr52) Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            Phospho-PFKP (Tyr52) Antibody (PA5-106229) in WB

            Western blot analysis of Phospho-PFKP (Tyr52) in various samples (Lane 1: rat brain lysate, Lane 2: rat muscle lysate, Lane 3: rat muscle lysates treated with blocking peptide). Samples were incubated with Phospho-PFKP (Tyr52) polyclonal antibody (Product # PA5-106229).
            View Product

            Phospho-PFKP (Tyr52) Antibody in Western Blot (WB)

            Product Details

            PA5-106229

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:100-1:3,000
            -
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            A synthesized peptide derived from human PFKP(Accession Q01813), corresponding to amino acid residues around phosphorylated Tyr52.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            sequential chromatography

            Storage buffer

            PBS, pH 7.4, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2817627

            Product Specific Information

            Antibody detects endogenous levels of PFKP only when phosphorylated at Y52.

            Target Information

            Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Bioinformatics

            Protein Aliases: 6-phosphofructo-1-kinase, C-type; 6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; ATP-dependent 6-phosphofructokinase, platelet type; ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255|HAMAP-Rule:MF_03184}; ATP-PFK; ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}; ATP:D-fructose 6-phosphate 1-phosphotransferase; EC 2.7.1.11; K6PP; PFK 1; PFK-C; PFK1; Phosphofructo-1-kinase isozyme C; Phosphofructokinase; phosphofructokinase 1; Phosphofructokinase, platelet type; Phosphohexokinase; phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}

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            Gene Aliases: 1200015H23Rik; 9330125N24Rik; ATP-PFK; PFK-C; PFK-P; Pfkc; PFKF; PFKP

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            UniProt ID: (Human) Q01813, (Rat) P47860, (Mouse) Q9WUA3

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            Entrez Gene ID: (Human) 5214, (Rat) 60416, (Mouse) 56421

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            Function(s)

            6-phosphofructokinase activity ATP binding protein complex binding metal ion binding cadherin binding involved in cell-cell adhesion fructose-6-phosphate binding nucleotide binding catalytic activity kinase activity transferase activity carbohydrate kinase kinase transferase metabolite interconversion enzyme

            Process(es)

            fructose 6-phosphate metabolic process canonical glycolysis cell-cell adhesion mitophagy in response to mitochondrial depolarization glycolytic process fructose 1,6-bisphosphate metabolic process carbohydrate phosphorylation protein homotetramerization metabolic process phosphorylation
            It has to be done as per old AB suggested Products section.
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