Invitrogen

Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Recombinant Superclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (4) RSK Pan antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

711336

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/218/223
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633010

Product Specific Information

This antibody is predicted to react with Monkey, Rat and Mouse

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

The p90 ribosomal S6 kinases (RSKs) comprise a family of serine/threonine kinases that lie at the terminus of the ERK pathway. In humans, the RSK family consists of four isoforms (RSK1 to-4). RSK family members are unusual among serine/threonine kinases in that they contain two distinct kinase domains, both of which are catalytically functional. Theses kinase domains are activated in a sequential manner by a series of phosphorylations. RSK regulates gene expression via association and phosphorylation of transcriptional regulators including c-Fos, estrogen receptor, NFkappaB/IkappaB alpha, cAMP-response element-binding protein (CREB). ERK activates the C-terminal kinase of RSK, leading to activation of the N-terminal kinase. Members of the RSK family are present in the cytoplasm as well as the nucleus. Addition of growth factor to the cells results in the activation of both cytosolic and nuclear RSK and the translocation of the cytosolic RSK into the nucleus upon activation. The activation and nuclear translocation of RSK result in phosphorylation and activation of transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 2; 90 kDa ribosomal protein S6 kinase 3; 90 kDa ribosomal protein S6 kinase 4; 90 kDa ribosomal protein S6 kinase 6; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1b; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1b; MAPK-activated protein kinase 1c; MAPKAP kinase 1a; MAPKAP kinase 1b; MAPKAP kinase 1C; MAPKAPK-1a; MAPKAPK-1b; MAPKAPK1D; mitogen- and stress-activated protein kinase 2; mitogen-activated protein kinase-activated protein kinase 1C; Nuclear mitogen- and stress-activated protein kinase 2; p90 rsk; p90-RSK 1; p90-RSK 2; p90-RSK 3; p90-RSK 6; p90RSK1; p90RSK6; p90S6K; pp90RSK2; pp90RSK3; pp90RSK4; Ribosomal protein kinase B; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-2; Ribosomal protein S6 kinase alpha-3; Ribosomal protein S6 kinase alpha-4; Ribosomal protein S6 kinase alpha-6; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 3; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 6; Ribosomal S6 kinase 1; Ribosomal S6 kinase 2; Ribosomal S6 kinase 3; Ribosomal S6 kinase 4; RP11-393H10.3; RSK-1; RSK-2; RSK-3; RSK-4; RSKB; S6K-alpha 1; S6K-alpha 6; S6K-alpha-1; S6K-alpha-2; S6K-alpha-3; S6K-alpha-4; S6K-alpha-6

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Gene Aliases: CLS; HU-1; HU-2; HU-3; ISPK-1; ISPK1; MAPKAPK1A; MAPKAPK1B; MAPKAPK1C; MRX19; MSK2; p90-RSK2; p90-RSK3; p90Rsk; p90RSK2; pp90RSK2; pp90RSK3; PP90RSK4; RPS6KA1; RPS6KA2; RPS6KA3; RPS6KA4; RPS6KA6; RSK; RSK-B; RSK1; RSK2; RSK3; RSK4; S6K-alpha; S6K-alpha-4; S6K-alpha2; S6K-alpha3

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UniProt ID: (Human) Q15418, (Human) Q15349, (Human) P51812, (Human) O75676, (Human) Q9UK32

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Entrez Gene ID: (Human) 6195, (Human) 6196, (Human) 6197, (Human) 8986, (Human) 27330

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Function(s)
magnesium ion binding protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process ribosomal protein S6 kinase activity protein kinase activity protein kinase binding histone kinase activity (H3-S10 specific) mitogen-activated protein kinase p38 binding protein modifying enzyme non-receptor serine/threonine protein kinase
Process(es)
protein phosphorylation apoptotic process cell cycle signal transduction positive regulation of cell growth intracellular signal transduction negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of translation in response to stress regulation of DNA-templated transcription in response to stress positive regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter hepatocyte proliferation positive regulation of hepatic stellate cell activation oocyte maturation brain renin-angiotensin system heart development negative regulation of cell proliferation positive regulation of gene expression cardiac muscle cell apoptotic process peptidyl-serine phosphorylation positive regulation of apoptotic process negative regulation of cell cycle negative regulation of meiotic nuclear division heart contraction regulation of protein processing cellular response to carbohydrate stimulus skeletal system development toll-like receptor signaling pathway central nervous system development response to lipopolysaccharide negative regulation of cytokine production regulation of transcription, DNA-templated inflammatory response histone phosphorylation positive regulation of CREB transcription factor activity positive regulation of histone phosphorylation positive regulation of histone acetylation histone H3-S10 phosphorylation histone H3-S28 phosphorylation positive regulation of NF-kappaB transcription factor activity interleukin-1-mediated signaling pathway DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator negative regulation of embryonic development negative regulation of ERK1 and ERK2 cascade negative regulation of mesoderm development
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