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Invitrogen

Phospho-RUNX2 (Ser275) Polyclonal Antibody

View all (47) RUNX2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

Application

Phospho-RUNX2 (Ser275) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Phospho-RUNX2 (Ser275) Antibody (PA5-105642) in ICC/IF

Immunocytochemistry analysis of Phospho-RUNX2 (Ser275) in HepG2 cells (serum starvation). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-RUNX2 (Ser275) polyclonal antibody (Product # PA5-105642) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ... View More

Phospho-RUNX2 (Ser275) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-RUNX2 (Ser275) Antibody in Western Blot (WB)

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Product Details

PA5-105642

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RUNX2(Accession Q13950), corresponding to amino acid residues around phosphorylated Ser275.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2817070

Product Specific Information

Antibody detects endogenous levels of RUNX2 only when phosphorylated at Ser275.

Target Information

RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acute myeloid leukemia 3 protein; AKV core binding factor; AML-3; CBF-alpha-1; core binding factor alpha 1; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; Oncogene AML-3; OSF-2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2 alpha A; PEBP2-alpha A; Polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; Runt-related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit

Gene Aliases: AML3; Cbf; CBF-alpha-1; Cbfa-1; CBFA1; CCD; CCD1; CLCD; LS3; OSF-2; OSF2; PEA2aA; PEBP2A; Pebp2a1; PEBP2aA; Pebpa2a; RUNX2

UniProt ID: (Human) Q13950, (Mouse) Q08775

Entrez Gene ID: (Human) 860, (Rat) 367218, (Mouse) 12393

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding ATP binding protein domain specific binding histone deacetylase binding bHLH transcription factor binding repressing transcription factor binding DNA binding transcription factor binding transcription regulatory region DNA binding DNA-binding transcription factor immunoglobulin fold transcription factor Runt transcription factor gene-specific transcriptional regulator

Process(es)

ossification osteoblast differentiation endochondral ossification osteoblast fate commitment chondrocyte differentiation chondrocyte development osteoblast development regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation hemopoiesis neuron differentiation T cell differentiation BMP signaling pathway positive regulation of chondrocyte differentiation embryonic forelimb morphogenesis regulation of fibroblast growth factor receptor signaling pathway odontogenesis of dentin-containing tooth regulation of odontogenesis of dentin-containing tooth cellular response to fibroblast growth factor stimulus regulation of cell differentiation positive regulation of osteoblast differentiation negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated cell maturation embryonic cranial skeleton morphogenesis stem cell differentiation cellular response to BMP stimulus positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus skeletal system development transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of gene expression regulation of ossification regulation of osteoblast differentiation positive regulation of ossification positive regulation of transcription from RNA polymerase II promoter skeletal system morphogenesis

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