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Application
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Phospho-SP1 (Thr739) Antibody (PA5-104771) in ICC/IF
Immunofluorescent analysis of Phospho-SP1 (Thr739) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-SP1 (Thr739) polyclonal antibody (Product # PA5-104771) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (r... View More
Product Details
PA5-104771
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SP1(Accession P08047), corresponding to amino acid residues around phosphorylated Thr739.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816244
Product Specific Information
Antibody detects endogenous levels of SP1 only when phosphorylated at Threonine 739.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:11205; Specificity protein 1; trans-acting transcription factor 1; Transcription factor Sp1
Gene Aliases: 1110003E12Rik; AA450830; AI845540; SP1; Sp1-1; TSFP1
UniProt ID: (Human) P08047, (Mouse) O89090, (Rat) Q01714
Entrez Gene ID: (Human) 6667, (Mouse) 20683, (Rat) 24790
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