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Phospho-SSH3 (Ser9) Antibody (PA5-105682) in ICC/IF
Immunocytochemistry analysis of Phospho-SSH3 (Ser9) in HepG2 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-SSH3 (Ser9) polyclonal antibody (Product # PA5-105682) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ant... View More
Product Details
PA5-105682
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SSH3(Accession Q8TE77), corresponding to amino acid residues around phosphorylated Ser9.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817110
Product Specific Information
Antibody detects endogenous levels of SSH3 only when phosphorylated at Ser9.
Target Information
The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hSSH-3L; Protein phosphatase Slingshot homolog 3; slingshot 3; slingshot homolog 3; slingshot-3L; slingshot-like 3; SSH-3L; SSH-like protein 3
Gene Aliases: BC028922; mSSH-3L; SSH-3; SSH3; SSH3L
UniProt ID: (Human) Q8TE77, (Mouse) Q8K330
Entrez Gene ID: (Human) 54961, (Mouse) 245857
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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