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Invitrogen
Phospho-VEGF Receptor 2 (Tyr951) Polyclonal Antibody
Application
FIGURE: 1 / 3
Phospho-VEGF Receptor 2 (Tyr951) Antibody (PA5-104882) in ICC/IF
Immunofluorescent analysis of Phospho-VEGF Receptor 2 (Tyr951) in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Phospho-VEGF Receptor 2 (Tyr951) polyclonal antibody (Product # PA5-104882) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-104882
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KDR(Accession P35968), corresponding to amino acid residues around phosphorylated Tyr951.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816355
Product Specific Information
Antibody detects endogenous levels of VEGFR2 only when phosphorylated at Tyrosine 951.
Target Information
KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2); FLK1 kinase insert domain receptor (VEGF receptor 2); KDR; kinase insert domain protein receptor; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2
Gene Aliases: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2
UniProt ID: (Human) P35968, (Rat) O08775, (Mouse) P35918
Entrez Gene ID: (Human) 3791, (Rat) 25589, (Mouse) 16542
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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