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Invitrogen
Phospho-cGAS (Ser291) Polyclonal Antibody
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Application
FIGURE: 1 / 1
Phospho-cGAS (Ser291) Antibody (PA5-117261) in WB
Western blot analysis of Phospho-cGAS (Ser291) in extracts of 293T cells. Samples were incubated with Phospho-cGAS (Ser291) Polyclonal antibody (Product # PA5-117261) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. 293T cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg pe... View More
Product Details
PA5-117261
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S291 of mouse CGAS (NP_7755622)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.76 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901891
Product Specific Information
Positive Samples: 293T
Immunogen sequence: PGSPA
Target Information
cGAS (Cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of 2', 5' phosphodiester linkage at the GpA step and the formation of 3', 5' phosphodiester linkage at the ApG step. This produces c[G(2',5')pA(3'5')p] responses. cGAS also binds cytosolic DNA directly, leading to the activation and synthesis of cGAMP. Then, a second messenger binds to and activates TMEM173/STING thereby triggering type-I interferon production. cGAS is also involved in innate immune sensorship of infection, detection of DNA from bacteria, response to cellular stress, DNA damage, and regulation of cellular senescence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2'3'-cGAMP synthase; C6orf150; cGAMP synthase; Cyclic GMP-AMP synthase; h-cGAS; Mab-21 domain-containing protein 1; MB21D1; protein MB21D1
Gene Aliases: C6orf150; CGAS; E330016A19Rik; h-cGAS; MB21D1
UniProt ID: (Human) Q8N884, (Mouse) Q8C6L5
Entrez Gene ID: (Human) 115004, (Mouse) 214763
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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