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Invitrogen
Phospho-gamma-H2AX (Ser139) Recombinant Rabbit Monoclonal Antibody (BLR053F)
FIGURE: 1 / 7
Phospho-gamma-H2AX (Ser139) Antibody (MA5-44299) in ICC/IF
Immunocytochemistry analysis of Histone H2A.X in HeLa cells treated with etoposide. Samples were incubated with Histone H2A.X monoclonal antibody (Product # MA5-44300) at a dilution of 1:250 followed by HRP-conjugated goat anti-rabbit IgG and DAB.
Product Details
MA5-44299
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR053F
Immunogen
Peptide surrounding phosphorylated serine 140 of human histone H2AX
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
purified
Storage buffer
BBS, pH 8.2, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
2-8°C
Shipping conditions
Wet ice
RRID
AB_2926429
Product Specific Information
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Target Information
H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX is phosphorylated by kinases such as ataxia telangiectasia mutated (ATM) and ATM-Rad3-related (ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gamma H2A X; gamma H2A.X; gamma-H2A.X; gh2ax; H2A histone family, member X; H2a/x; H2AX histone; Histone H2A.X; Histone H2AX
Gene Aliases: H2A.X; H2A/X; H2AFX; H2AX
UniProt ID: (Human) P16104
Entrez Gene ID: (Human) 3014
DNA damage checkpoint
double-strand break repair via homologous recombination
double-strand break repair
double-strand break repair via nonhomologous end joining
nucleosome assembly
chromatin silencing
cellular response to DNA damage stimulus
spermatogenesis
response to ionizing radiation
viral process
cerebral cortex development
positive regulation of DNA repair
meiotic cell cycle
cellular response to gamma radiation
cellular senescence
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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