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Proteintech

Phospholemman/FXYD1 Polyclonal Antibody

32 References

View all (11) FXYD1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (14)

Application

Phospholemman/FXYD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 14

Phospholemman/FXYD1 Antibody (13721-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using (Product # 13721-1-AP) (Phospholemman/FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Phospholemman/FXYD1 Antibody in Western Blot (WB)

Phospholemman/FXYD1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13721-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,500

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Not applicable (N/A)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospholemman/FXYD1 Fusion Protein Ag4669 (1-92 aa encoded by BC032800)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MASLGHILV FCVGLLTMAK AESPKEHDPF TYDYQSLQIG GLVIAGILFI LGILIVLSRR CRCKFNQQQR TGEPDEEEGT FRSSIRRLST RRR (1-92 aa encoded by BC032800)

Target Information

This gene encodes a member of the FXYD family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity and act as an accessory protein of Na,K-ATPase. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effect of sample fractionation and normalization when immunoblotting for human muscle Na⁺/K⁺-ATPase subunits and glycogen synthase.

Analytical biochemistry

Thomassen M,Bangsbo J,Hostrup M

Sat Apr 01 00:00:00 UTC 2023

Skeletal muscle phenotype and game performance in elite women football players.

Scandinavian journal of medicine & science in sports

Mohr M,Fatouros IG,Jamurtas AZ,Draganidis D,Thomassen M,Ørntoft C,Ermidis G,Loules G,Batsilas D,Poulios A,Papanikolaou K,Randers MB,Krustrup P,Nybo L

Fri Apr 01 00:00:00 UTC 2022

Effect of differentiation, de novo innervation, and electrical pulse stimulation on mRNA and protein expression of Na+,K+-ATPase, FXYD1, and FXYD5 in cultured human skeletal muscle cells.

PloS one

Jan V,Miš K,Nikolic N,Dolinar K,Petrič M,Bone A,Thoresen GH,Rustan AC,Marš T,Chibalin AV,Pirkmajer S

Fri Aug 20 00:00:00 UTC 2021

No additive effect of acetaminophen when co-ingested with caffeine on cycling performance in well-trained young men.

Journal of applied physiology (Bethesda, Md. : 1985)

Jessen S,Eibye K,Christensen PM,Hostrup M,Bangsbo J

Thu Jul 01 00:00:00 UTC 2021

Skeletal muscle proteins important for work capacity are altered with type 2 diabetes - Effect of 10-20-30 training.

Physiological reports

Baasch-Skytte T,Gunnarsson TP,Fiorenza M,Bangsbo J

Fri Jan 01 00:00:00 UTC 2021

Insulin-Like Growth Factor-1 Down-Regulates the Phosphorylation of FXYD1 and Rescues Behavioral Deficits in a Mouse Model of Rett Syndrome.

Frontiers in neuroscience

Yuan ZF,Mao SS,Shen J,Jiang LH,Xu L,Xu JL,Gao F

Mon Sep 28 00:00:00 UTC 2020

Fatigue-induced change in T-system excitability and its major cause in rat fast-twitch skeletal muscle in vivo.

The Journal of physiology

Watanabe D,Wada M

Sun Nov 01 00:00:00 UTC 2020

Isoform-specific Na,K-ATPase and membrane cholesterol remodeling in motor endplates in distinct mouse models of myodystrophy.

American journal of physiology. Cell physiology

Kravtsova VV,Bouzinova EV,Chibalin AV,Matchkov VV,Krivoi II

Fri May 01 00:00:00 UTC 2020

Cycling with blood flow restriction improves performance and muscle K⁺ regulation and alters the effect of anti-oxidant infusion in humans.

The Journal of physiology

Christiansen D,Eibye KH,Rasmussen V,Voldbye HM,Thomassen M,Nyberg M,Gunnarsson TGP,Skovgaard C,Lindskrog MS,Bishop DJ,Hostrup M,Bangsbo J

Wed May 01 00:00:00 UTC 2019

The effect of repeated periods of speed endurance training on performance, running economy, and muscle adaptations.

Scandinavian journal of medicine & science in sports

Skovgaard C,Almquist NW,Bangsbo J

Thu Feb 01 00:00:00 UTC 2018

The effect of repeated periods of speed endurance training on performance, running economy, and muscle adaptations.

Scandinavian journal of medicine & science in sports

Skovgaard C,Almquist NW,Bangsbo J

Thu Feb 01 00:00:00 UTC 2018

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 UTC 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 UTC 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 UTC 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 UTC 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 UTC 2020

Axial Tubule Junctions Activate Atrial Ca²⁺ Release Across Species.

Frontiers in physiology

Brandenburg S,Pawlowitz J,Fakuade FE,Kownatzki-Danger D,Kohl T,Mitronova GY,Scardigli M,Neef J,Schmidt C,Wiedmann F,Pavone FS,Sacconi L,Kutschka I,Sossalla S,Moser T,Voigt N,Lehnart SE

Tue Sep 29 00:00:00 UTC 2020

Effect of tapering after a period of high-volume sprint interval training on running performance and muscular adaptations in moderately trained runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Almquist NW,Kvorning T,Christensen PM,Bangsbo J

Thu Feb 01 00:00:00 UTC 2018

Effect of tapering after a period of high-volume sprint interval training on running performance and muscular adaptations in moderately trained runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Almquist NW,Kvorning T,Christensen PM,Bangsbo J

Thu Feb 01 00:00:00 UTC 2018

Cold-water immersion after training sessions: effects on fiber type-specific adaptations in muscle K⁺ transport proteins to sprint-interval training in men.

Journal of applied physiology (Bethesda, Md. : 1985)

Christiansen D,Bishop DJ,Broatch JR,Bangsbo J,McKenna MJ,Murphy RM

Wed Aug 01 00:00:00 UTC 2018

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.

Scientific reports

Su X,Liang H,Wang H,Chen G,Jiang H,Wu Q,Liu T,Liu Q,Yu T,Gu Y,Yang B,Shan H

Tue Apr 11 00:00:00 UTC 2017

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.

Scientific reports

Su X,Liang H,Wang H,Chen G,Jiang H,Wu Q,Liu T,Liu Q,Yu T,Gu Y,Yang B,Shan H

Tue Apr 11 00:00:00 UTC 2017

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.

Scientific reports

Su X,Liang H,Wang H,Chen G,Jiang H,Wu Q,Liu T,Liu Q,Yu T,Gu Y,Yang B,Shan H

Tue Apr 11 00:00:00 UTC 2017

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.

Scientific reports

Su X,Liang H,Wang H,Chen G,Jiang H,Wu Q,Liu T,Liu Q,Yu T,Gu Y,Yang B,Shan H

Tue Apr 11 00:00:00 UTC 2017

Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Almquist NW,Bangsbo J

Sun Jan 01 00:00:00 UTC 2017

Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Almquist NW,Bangsbo J

Sun Jan 01 00:00:00 UTC 2017

Distinct α2 Na,K-ATPase membrane pools are differently involved in early skeletal muscle remodeling during disuse.

The Journal of general physiology

Kravtsova VV,Petrov AM,Matchkov VV,Bouzinova EV,Vasiliev AN,Benziane B,Zefirov AL,Chibalin AV,Heiny JA,Krivoi II

Mon Feb 01 00:00:00 UTC 2016

Distinct α2 Na,K-ATPase membrane pools are differently involved in early skeletal muscle remodeling during disuse.

The Journal of general physiology

Kravtsova VV,Petrov AM,Matchkov VV,Bouzinova EV,Vasiliev AN,Benziane B,Zefirov AL,Chibalin AV,Heiny JA,Krivoi II

Mon Feb 01 00:00:00 UTC 2016

Muscle variables of importance for physiological performance in competitive football.

European journal of applied physiology

Mohr M,Thomassen M,Girard O,Racinais S,Nybo L

Mon Feb 01 00:00:00 UTC 2016

Muscle variables of importance for physiological performance in competitive football.

European journal of applied physiology

Mohr M,Thomassen M,Girard O,Racinais S,Nybo L

Mon Feb 01 00:00:00 UTC 2016

Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes.

American journal of physiology. Regulatory, integrative and comparative physiology

Thomassen M,Gunnarsson TP,Christensen PM,Pavlovic D,Shattock MJ,Bangsbo J

Fri Apr 01 00:00:00 UTC 2016

Effect of formoterol, a long-acting β2-adrenergic agonist, on muscle strength and power output, metabolism, and fatigue during maximal sprinting in men.

American journal of physiology. Regulatory, integrative and comparative physiology

Kalsen A,Hostrup M,Backer V,Bangsbo J

Wed Jun 01 00:00:00 UTC 2016

Effect of formoterol, a long-acting β2-adrenergic agonist, on muscle strength and power output, metabolism, and fatigue during maximal sprinting in men.

American journal of physiology. Regulatory, integrative and comparative physiology

Kalsen A,Hostrup M,Backer V,Bangsbo J

Wed Jun 01 00:00:00 UTC 2016

Phospholemman is not required for the acute stimulation of Na⁺-K⁺-ATPase α₂-activity during skeletal muscle fatigue.

American journal of physiology. Cell physiology

Manoharan P,Radzyukevich TL,Hakim Javadi H,Stiner CA,Landero Figueroa JA,Lingrel JB,Heiny JA

Tue Dec 15 00:00:00 UTC 2015

Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men.

Journal of applied physiology (Bethesda, Md. : 1985)

Hostrup M,Kalsen A,Onslev J,Jessen S,Haase C,Habib S,Ørtenblad N,Backer V,Bangsbo J

Tue Sep 01 00:00:00 UTC 2015

Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men.

Journal of applied physiology (Bethesda, Md. : 1985)

Hostrup M,Kalsen A,Onslev J,Jessen S,Haase C,Habib S,Ørtenblad N,Backer V,Bangsbo J

Tue Sep 01 00:00:00 UTC 2015

Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Christensen PM,Larsen S,Andersen TR,Thomassen M,Bangsbo J

Sat Nov 15 00:00:00 UTC 2014

Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners.

Journal of applied physiology (Bethesda, Md. : 1985)

Skovgaard C,Christensen PM,Larsen S,Andersen TR,Thomassen M,Bangsbo J

Sat Nov 15 00:00:00 UTC 2014

Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humans.

The Journal of physiology

Thomassen M,Murphy RM,Bangsbo J

Fri Mar 15 00:00:00 UTC 2013

Fibre type-specific change in FXYD1 phosphorylation during acute intense exercise in humans.

The Journal of physiology

Thomassen M,Murphy RM,Bangsbo J

Fri Mar 15 00:00:00 UTC 2013

Influence of chronic and acute spinal cord injury on skeletal muscle Na+-K+-ATPase and phospholemman expression in humans.

American journal of physiology. Endocrinology and metabolism

Boon H,Kostovski E,Pirkmajer S,Song M,Lubarski I,Iversen PO,Hjeltnes N,Widegren U,Chibalin AV

Sun Apr 01 00:00:00 UTC 2012

Influence of chronic and acute spinal cord injury on skeletal muscle Na+-K+-ATPase and phospholemman expression in humans.

American journal of physiology. Endocrinology and metabolism

Boon H,Kostovski E,Pirkmajer S,Song M,Lubarski I,Iversen PO,Hjeltnes N,Widegren U,Chibalin AV

Sun Apr 01 00:00:00 UTC 2012

Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.

PloS one

Chibalin AV,Heiny JA,Benziane B,Prokofiev AV,Vasiliev AV,Kravtsova VV,Krivoi II

Mon Jul 23 00:00:00 UTC 2012

Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.

PloS one

Chibalin AV,Heiny JA,Benziane B,Prokofiev AV,Vasiliev AV,Kravtsova VV,Krivoi II

Mon Jul 23 00:00:00 UTC 2012

Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Benziane B,Widegren U,Pirkmajer S,Henriksson J,Stepto NK,Chibalin AV

Thu Sep 01 00:00:00 UTC 2011

Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Benziane B,Widegren U,Pirkmajer S,Henriksson J,Stepto NK,Chibalin AV

Thu Sep 01 00:00:00 UTC 2011

Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Benziane B,Widegren U,Pirkmajer S,Henriksson J,Stepto NK,Chibalin AV

Thu Sep 01 00:00:00 UTC 2011

Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Benziane B,Widegren U,Pirkmajer S,Henriksson J,Stepto NK,Chibalin AV

Thu Sep 01 00:00:00 UTC 2011

The nicotinic acetylcholine receptor and the Na,K-ATPase alpha2 isoform interact to regulate membrane electrogenesis in skeletal muscle.

The Journal of biological chemistry

Heiny JA,Kravtsova VV,Mandel F,Radzyukevich TL,Benziane B,Prokofiev AV,Pedersen SE,Chibalin AV,Krivoi II

Fri Sep 10 00:00:00 UTC 2010

The nicotinic acetylcholine receptor and the Na,K-ATPase alpha2 isoform interact to regulate membrane electrogenesis in skeletal muscle.

The Journal of biological chemistry

Heiny JA,Kravtsova VV,Mandel F,Radzyukevich TL,Benziane B,Prokofiev AV,Pedersen SE,Chibalin AV,Krivoi II

Fri Sep 10 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: FXYD domain-containing ion transport regulator 1; Phospholemman; Sodium Potassium ATPase; Sodium/potassium-transporting ATPase subunit FXYD1

Gene Aliases: 0610012C17Rik; 1110006M24Rik; FXYD1; PLM; Pml

UniProt ID: (Human) O00168, (Mouse) Q9Z239, (Rat) O08589

Entrez Gene ID: (Human) 5348, (Mouse) 56188, (Rat) 58971

Function(s)

chloride channel activity sodium channel regulator activity ion channel binding ion channel activity myosin binding ATPase binding protein binding primary active transporter

Process(es)

chloride transport muscle contraction regulation of heart contraction ion transmembrane transport regulation of cardiac muscle cell membrane potential chloride transmembrane transport positive regulation of sodium ion export from cell regulation of cardiac conduction regulation of sodium ion transmembrane transporter activity transport ion transport positive regulation of organic acid transport regulation of cytosolic calcium ion concentration regulation of cardiac muscle cell contraction positive regulation of inorganic anion transmembrane transport brain development

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Phospholemman/FXYD1 Polyclonal Antibody

Phospholemman/FXYD1 Antibody (13721-1-AP) in IHC (P)

Product Details

13721-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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