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Invitrogen
Phosphoserine/threonine Monoclonal Antibody (7A1P3)
Application
FIGURE: 1 / 1
Phosphoserine/threonine Antibody (MA5-38234) in WB
Western blot analysis of Phosphoserine/threonine in extracts of Jurkat cells. Samples were incubated with Phosphoserine/threonine Monoclonal antibody (Product # MA5-38234) using a dilution of 1:500, followed by HRP Goat Anti-Mouse IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. Lysates/proteins: 25 µg per lane. Blocking buffer... View More
Product Details
MA5-38234
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
7A1P3
Immunogen
A synthetic peptide corresponding to a sequence containing phosphorylated Serine/Threonine
Conjugate
Unconjugated
Form
Liquid
Concentration
1.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898150
Product Specific Information
Positive Samples: Jurkat
Target Information
A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. The (Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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