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Rockland
Phosphotyrosine Monoclonal Antibody (13F9)
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Application
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Phosphotyrosine Antibody (500-301-262) in WB
Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after Coomassie staining. Load: 10 µg per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody: IRDye™800 Conjugated Goat-a-Mouse IgG [H&L] (610-132-120) diluted 1:2,500 for 30 at room temperature. Block: 5% BLOTTO overnight at 4°C. Other band(s): phosphotyrosine splice variants and isoforms.
Product Details
500-301-262
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
13F9
Immunogen
phosphotyrosine conjugated KLH
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This product is clarified ascites produced in balb/c mice using clone 13F9. Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.
Target Information
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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