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Invitrogen
Phycoerythrin Monoclonal Antibody (eBioPE-DLF), eBioscience™
Application
FIGURE: 1 / 1
Phycoerythrin Antibody (14-4120-81) in Flow
Staining of normal human peripheral blood cells with Rat Anti-Human/Mouse CD44 PE (Product # 12-0441-82), followed by 0.125 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 0.125 µg of Anti-Phycoerythrin Purified (right), followed by detection with Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.
Product Details
14-4120-81
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioPE-DLF
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_529613
Product Specific Information
Description: The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested.
Applications Reported: This eBioPE-DLF antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioPE-DLF antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-RPE (R-Phycoerythrin) antibodies can be used to bind conjugated primary antibody without decreasing fluorescent intensity of the RPE fluorochrome. This method is used for separation of cells labeled with RPE-conjugated antibodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PE; R-PE; R-phycoerythrin; RPE
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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