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Proteintech

Plakophilin 2 Polyclonal Antibody

View all (14) PKP2 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (4)

Application

Plakophilin 2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Plakophilin 2 Antibody (26479-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Plakophilin 2 antibody (26479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (SA00013-2).

Plakophilin 2 Antibody in Immunocytochemistry (ICC/IF)

Plakophilin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Plakophilin 2 Antibody in Western Blot (WB)

Plakophilin 2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Plakophilin 2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Plakophilin 2 antibody (26479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (SA00013-2).

Plakophilin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Plakophilin 2 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 26479-1-AP (Plakophilin 2 antibody) at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

Plakophilin 2 Antibody in Immunoprecipitation (IP) — Immunoprecipitation result of anti-Plakophilin 2 (IP: 26479-1-AP, 4 µg; Detection: 26479-1-AP 1:1000) with HepG2 cells lysate 2200 µg.

Product Details

26479-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Immunocytochemistry (ICC/IF)

1:200-1:800

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

0.5-4.0 µg for 1.0-3.0 mg of total protein lysate

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Plakophilin 2 fusion protein Ag24155

Conjugate

Unconjugated

Form

Liquid

Concentration

0.75 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Immunogen sequence: MAAPGAPAEY GYIRTVLGQQ ILGQLDSSSL ALPSEAKLKL AGSSGRGGQT VKSLRIQEQV QQTLARKGRS SVGNGNLHRT SSVPEYVYNL HLVENDFVGG RSPVPKTYDM LKAGTTATYE GRWGRGTAQY SSQKSVEERS LRHPLRRLEI SPDSSPERAH YTHSDYQYSQ RSQAGHTLHH QESRRAALLV PPRYARSEIV GVSRAGTTSR QRHFDTYHRQ YQHGSVSDTV FDSIPANPAL LTYPRPGTSR SMGNLLEKEN YLTAGLTVGQ VRPLVPLQPV TQNRASRSSW HQSSFHSTRT LREAGPSVAV DSSGRRAHLT VGQAAAGGSG NLLTERSTFT DSQLGNADME M

Target Information

Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 1 mediates increases in desmosomal protein content, desmosome assembly and regulation of cell migration. Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin 4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.

Journal of neuro-oncology

Xu H,Cao Y,Ruan J,Wang F,He Y,Yang L,Yu T,Du F,Zhang N,Cao X

Tue Oct 01 00:00:00 UTC 2024

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.

Journal of neuro-oncology

Xu H,Cao Y,Ruan J,Wang F,He Y,Yang L,Yu T,Du F,Zhang N,Cao X

Tue Oct 01 00:00:00 UTC 2024

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.

Journal of neuro-oncology

Xu H,Cao Y,Ruan J,Wang F,He Y,Yang L,Yu T,Du F,Zhang N,Cao X

Tue Oct 01 00:00:00 UTC 2024

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.

Journal of neuro-oncology

Xu H,Cao Y,Ruan J,Wang F,He Y,Yang L,Yu T,Du F,Zhang N,Cao X

Tue Oct 01 00:00:00 UTC 2024

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.

Journal of neuro-oncology

Xu H,Cao Y,Ruan J,Wang F,He Y,Yang L,Yu T,Du F,Zhang N,Cao X

Tue Oct 01 00:00:00 UTC 2024

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.

Journal of neuro-oncology

Xu H,Cao Y,Ruan J,Wang F,He Y,Yang L,Yu T,Du F,Zhang N,Cao X

Tue Oct 01 00:00:00 UTC 2024

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.

Journal of neuro-oncology

Xu H,Cao Y,Ruan J,Wang F,He Y,Yang L,Yu T,Du F,Zhang N,Cao X

Tue Oct 01 00:00:00 UTC 2024

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.

Journal of neuro-oncology

Xu H,Cao Y,Ruan J,Wang F,He Y,Yang L,Yu T,Du F,Zhang N,Cao X

Tue Oct 01 00:00:00 UTC 2024

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

Journal of nanobiotechnology

Lai X,Wang M,Zhang Z,Chen S,Tan X,Liu W,Liang H,Li L,Shao L

Wed Jun 05 00:00:00 UTC 2024

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

Journal of nanobiotechnology

Lai X,Wang M,Zhang Z,Chen S,Tan X,Liu W,Liang H,Li L,Shao L

Wed Jun 05 00:00:00 UTC 2024

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

Journal of nanobiotechnology

Lai X,Wang M,Zhang Z,Chen S,Tan X,Liu W,Liang H,Li L,Shao L

Wed Jun 05 00:00:00 UTC 2024

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

Journal of nanobiotechnology

Lai X,Wang M,Zhang Z,Chen S,Tan X,Liu W,Liang H,Li L,Shao L

Wed Jun 05 00:00:00 UTC 2024

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

Journal of nanobiotechnology

Lai X,Wang M,Zhang Z,Chen S,Tan X,Liu W,Liang H,Li L,Shao L

Wed Jun 05 00:00:00 UTC 2024

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

Journal of nanobiotechnology

Lai X,Wang M,Zhang Z,Chen S,Tan X,Liu W,Liang H,Li L,Shao L

Wed Jun 05 00:00:00 UTC 2024

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

Journal of nanobiotechnology

Lai X,Wang M,Zhang Z,Chen S,Tan X,Liu W,Liang H,Li L,Shao L

Wed Jun 05 00:00:00 UTC 2024

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

Journal of nanobiotechnology

Lai X,Wang M,Zhang Z,Chen S,Tan X,Liu W,Liang H,Li L,Shao L

Wed Jun 05 00:00:00 UTC 2024

Bioinformatics

Protein Aliases: Plakophilin-2

Gene Aliases: ARVD9; PKP2

UniProt ID: (Human) Q99959, (Rat) F1M7L9

Entrez Gene ID: (Human) 5318, (Rat) 287925

Function(s)

molecular_function protein kinase C binding protein binding sodium channel regulator activity intermediate filament binding protein complex scaffold ion channel binding alpha-catenin binding cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication cadherin binding involved in cell-cell adhesion intermediate filament binding protein

Process(es)

desmosome assembly heart development negative regulation of cell proliferation positive regulation of sodium ion transport gap junction assembly single organismal cell-cell adhesion negative regulation of cell migration adherens junction maintenance intermediate filament bundle assembly maintenance of organ identity ventricular cardiac muscle tissue morphogenesis lipid homeostasis cardiac muscle cell action potential cardiac muscle cell action potential involved in contraction ventricular cardiac muscle cell action potential cell-cell signaling involved in cardiac conduction cell communication by electrical coupling involved in cardiac conduction bundle of His cell-Purkinje myocyte adhesion involved in cell communication regulation of heart rate by cardiac conduction establishment of protein localization to plasma membrane regulation of ventricular cardiac muscle cell action potential regulation of bicellular tight junction assembly bundle of His cell to Purkinje myocyte communication

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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