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Invitrogen
Plectin Polyclonal Antibody
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Application
FIGURE: 1 / 6
Plectin Antibody (PA5-79829) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Plectin in U20S cells using Plectin Polyclonal Antibody (Product # PA5-79829). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter... View More
Product Details
PA5-79829
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Plectin (2644-2671aa RFIEQEKAKLEQLFQDEVAKAQQLREEQ).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746944
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell. IHC: human intestine cancer tissue, human lung cancer tissue, mouse intestine tissue, rat intestine tissue. ICC/IF: U20S cell.
Target Information
Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes. Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans. The expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development and it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms which carry out distinct and specific functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HD1; Hemidesmosomal protein 1; PCN; Plectin; plectin 1; plectin 1, intermediate filament binding protein 500kDa; Plectin-1; Plectin-6
Gene Aliases: AA591047; AU042537; EBS1; EBSMD; EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; PCN; PLEC; PLEC1; PLEC1b; PLTN
UniProt ID: (Human) Q6S380, (Mouse) Q9QXS1
Entrez Gene ID: (Human) 5339, (Rat) 64204, (Mouse) 18810
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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