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            • Primary Antibodies ›
            • Prolactin Receptor Antibodies

            Invitrogen

            Prolactin Receptor Monoclonal Antibody (1A2B1)

            Advanced Verification
            34 Published Figures
            46 References
            View all (36) Prolactin Receptor antibodies
            Datasheet
            Protocols
            Questions & Answers
            Promotion Available
            Learn more
            Catalog # 35-9200
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            Price (USD)
            371.65
            Online Exclusive
            495.00
            Save 123.35 (25%)
            100 µg
             
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            Datasheet
            Protocols
            Questions & Answers

            Cite Prolactin Receptor Monoclonal Antibody (1A2B1)

            • Antibody Testing Data (2)
            • Published Figures (34)
            • Advanced Verification (1)
            Application
            Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 37

            Prolactin Receptor Antibody (35-9200) in ICC/IF

            Immunofluorescence analysis of Prolactin Receptor was performed using 70% confluent log phase SW40 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Prolactin Receptor (ECD) (1A2B1) Monoclonal Antibody (Product # 35-9200) at 2µg/mL in 0.1% BSA and incub... View More
            View Product

            Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
            Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
            Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
            Prolactin Receptor Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            Prolactin Receptor Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
            Prolactin Receptor Antibody in Immunohistochemistry (IHC)
            Prolactin Receptor Antibody in Immunohistochemistry (IHC)
            Prolactin Receptor Antibody in Immunohistochemistry (IHC)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot, Flow Cytometry (WB, Flow)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot, Immunoprecipitation (WB, IP)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot, Immunoprecipitation (WB, IP)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Western Blot (WB)
            Prolactin Receptor Antibody in Proximity Ligation Assay (PLA) (PLA)
            Prolactin Receptor Antibody

            Product Details

            35-9200

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1-3 µg/mL
            View 21 publications 21 publications

            Immunohistochemistry (IHC)

            5-10 µg/mL
            View 8 publications 8 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            -
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            2 µg/mL
            View 5 publications 5 publications

            Flow Cytometry (Flow)

            -
            View 2 publications 2 publications

            Immunoprecipitation (IP)

            5-10 µg/mL
            View 3 publications 3 publications

            Neutralization (Neu)

            -
            View 1 publication 1 publication

            in situ PLA (PLA)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 4 publications 4 publications
            Product Specifications

            Species Reactivity

            Human

            Published species

            Hamster, Human, Mouse

            Host/Isotype

            Mouse / IgG2b, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            1A2B1

            Immunogen

            Recombinant human prolactin receptor protein containing the extracellular domain (ECD).

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.4

            Contains

            0.1% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2533231

            Product Specific Information

            This antibody is predicted to react with mouse and rat and reacts with the long form of the human prolactin receptor (hPRLr) protein at ~85-90 kDa, and may also identify the human prolactin receptor intermediate and delta S1 isoforms by western blotting. On immunoprecipitation, this antibody strongly recognizes only the long form of hPRLr. During development reactivity was confirmed with human T-47D breast carcinoma cells.

            Target Information

            Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Related Products for

            Bioinformatics

            Protein Aliases: hPRL receptor; Prl R; PRL-R; Prolactin receptor; secreted prolactin binding protein

            View more View less

            Gene Aliases: HPRL; hPRLrI; MFAB; PRLR

            View more View less

            UniProt ID: (Human) P16471

            View more View less

            Entrez Gene ID: (Human) 5618

            View more View less

            Function(s)

            prolactin receptor activity protein binding peptide hormone binding protein homodimerization activity ornithine decarboxylase activator activity metal ion binding transmembrane signal receptor

            Process(es)

            steroid biosynthetic process cell surface receptor signaling pathway activation of transmembrane receptor protein tyrosine kinase activity embryo implantation lactation regulation of cell adhesion regulation of epithelial cell differentiation prolactin signaling pathway T cell activation activation of JAK2 kinase activity negative regulation of apoptotic process JAK-STAT cascade involved in growth hormone signaling pathway mammary gland epithelial cell differentiation prostate gland growth mammary gland alveolus development
            It has to be done as per old AB suggested Products section.
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