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Invitrogen
Protein APC Polyclonal Antibody
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Application
FIGURE: 1 / 5
Protein APC Antibody (PA5-30580) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Protein APC was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Protein APC Polyclonal Antibody (Product # PA5 30580) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-30580
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human APC. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.44 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548054
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adenomatosis polyposis coli; adenomatosis polyposis coli tumor suppressor; Adenomatous polyposis coli protein; Deleted in polyposis 2.5; FAP; FPC; multiple intestinal neoplasia; Protein APC; protein phosphatase 1, regulatory subunit 46; truncated adenomatosis polyposis coli; WNT signaling pathway regulator
Gene Aliases: AI047805; APC; AU020952; AW124434; BTPS2; CC1; DP2; DP2.5; DP3; GS; mAPC; Min; PPP1R46; RATAPC
UniProt ID: (Human) P25054, (Rat) P70478
Entrez Gene ID: (Human) 324, (Mouse) 11789, (Rat) 24205
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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