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Bethyl Laboratories
Pur-alpha Polyclonal Antibody
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Application
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Pur-alpha Antibody (A303-544A) in IP
Detection of human Pur-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pur-alpha antibody (Product # A303-544A) used for IP at 6 µg/mg lysate. Pur-alpha was less efficiently immunoprecipitated by rabbit anti-Pur-alpha antibody A303-543A, which recognizes an upstream epitope. For blotting immunoprecipitated Pur-alpha, A303-543A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A303-544A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 and 150 of Human Purine-Rich Element Binding Protein Alpha.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: purine-rich element binding protein A; Purine-rich single-stranded DNA-binding protein alpha; Transcriptional activator protein Pur-alpha
Gene Aliases: MRD31; PUR-ALPHA; PUR1; PURA; PURALPHA
UniProt ID: (Human) Q00577
Entrez Gene ID: (Human) 5813
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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