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Application
FIGURE: 1 / 1
RAB3D Antibody (MA5-45970) in WB
Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of RAB3D protein. Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney. Load: 15 µg. Blocking: 5% Skim Milk Powder in TBST. Samples were incubated with RAB3D monoclonal antibody (Product # MA5-45970) at 1:1,000 for 2 hours at RT with shaking, followed by Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at RT with shaking. Color Development: Chemil... View More
Product Details
MA5-45970
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5E9
Immunogen
Full length recombinant RAB3D protein
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932424
Product Specific Information
A 1:1,000 dilution of MA5-45970 was sufficient for detection of RAB3D in 10 µg of A549, T-47D cells, mouse and rat brain, kidney by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.|Detects approximately 24 kDa. Does not show cross reactivity to RAB3A, RAB3B, or RAB3C.
Target Information
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glioblastoma overexpressed; GTP-binding protein Rab-3D; Rab3D upregulated with myeloid differentiation; Ras-related protein Rab-3D
Gene Aliases: C130057E11Rik; D2-2; GOV; RAB16; RAB3D; RAD3D
UniProt ID: (Human) O95716, (Mouse) P35276, (Rat) Q63942
Entrez Gene ID: (Human) 9545, (Mouse) 19340, (Rat) 140665
exocytosis
small GTPase mediated signal transduction
protein transport
peptidyl-cysteine methylation
bone resorption
positive regulation of regulated secretory pathway
transport
intracellular protein transport
vesicle docking involved in exocytosis
protein secretion
synaptic vesicle exocytosis
regulation of exocytosis
Rab protein signal transduction
protein localization to plasma membrane
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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