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Invitrogen
RAB5 Polyclonal Antibody
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Application
FIGURE: 1 / 13
RAB5 Antibody (PA5-29022) in ICC/IF
MRC-5 cells (fetal lung fibroblasts) were grown on glass coverslips in 6 well dishes and infected for 48 h with VZV. Cells were fixed in 2% Paraformaldehyde and permeabilized with 0.02% Triton X-100 for 1 h at room temperature. Blocked cells for 2 h at RT in 5% non-fat milk plus 2.5% normal goat serum in PBS. Anti-VZV gE mouse monoclonal antibody (red) and anti-Rab5 rabbit polyclonal antibody (green, Product # PA5-29022, 1:100 in PBS) were incubated 2 h at RT (with rocking), then overnight at 4oC (with gentle rocking). After washing with P... View More
Product Details
PA5-29022
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RAB5A. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1.47 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2546498
Product Specific Information
Recommended positive controls: A431, Raji, mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Rhesus Monkey (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RAB5 are among the small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Rab 5; Rab 5A; Ras related protein; RAS-associated protein RAB5A; Ras-related protein Rab-5A; Small GTP-binding protein rab5
Gene Aliases: 2410015H04Rik; AI663973; AU021172; nnyRab5a; RAB5; RAB5A
UniProt ID: (Human) P20339, (Mouse) Q9CQD1, (Rat) M0RC99
Entrez Gene ID: (Human) 5868, (Mouse) 271457, (Rat) 64633
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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