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Invitrogen
RAB5A Monoclonal Antibody (2E8B11), eBioscience™
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
RAB5A Antibody (14-9711-82) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed for detection of endogenous Rab5a using Anti-Rab5a Monoclonal Antibody (Product # 14-9711-82, 1:100 dilution) and labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000). Panel a) shows representative cells that were stained for detection of Rab5a protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin ... View More
Product Details
14-9711-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2E8B11
Immunogen
Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884043
Product Specific Information
Description: This 2E8B11 monoclonal antibody recognizes human Rab5a, which is a GTPase involved in intracellular membrane trafficking. Rab5a is reported to have ubiquitous expression.
Applications Reported: The 2E8B11 clone has been reported for use in intracellular flow cytometry, western blot, ELISA, immunocytochemistry of cells and immunohistochemistry of tissue.
Applications Tested: This 2E8B11 antibody has been tested by western blot in multiple human cell lines.
Applications Tested: This 2E8B11 antibody has been tested by immunocytochemical staining of fixed and permeabilized HeLa cells.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
RAB5 are among the small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Rab 5; Rab 5A; Ras related protein; RAS-associated protein RAB5A; Ras-related protein Rab-5A
Gene Aliases: RAB5; RAB5A
UniProt ID: (Human) P20339
Entrez Gene ID: (Human) 5868
endocytosis
phagocytosis
small GTPase mediated signal transduction
blood coagulation
protein transport
regulation of endocytosis
synaptic vesicle recycling
early endosome to late endosome transport
positive regulation of exocytosis
regulation of endosome size
regulation of filopodium assembly
receptor internalization involved in canonical Wnt signaling pathway
regulation of synaptic vesicle exocytosis
regulation of autophagosome assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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