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Application
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RACK1 Antibody (A302-545A) in WB
Detection of human RACK1 by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, HeLa, Jurkat, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RACK1 antibody (Product # A302-545A lot 3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A302-545A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 and 50 of Human receptor of activated protein kinase C 1.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken, Bovine and Pig
Target Information
RACK1 (receptor for activated C kinase 1) was identified through its binding to various PKC isoforms. Its main function is to recruit PKC and various other proteins to specific locations to form multiprotein complexes, mediating various signal pathways. RACK1 is required for VANGL2 membrane localization and for PTK2/FAK1 phosphorylation and dephosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12-3; activated protein kinase C receptor; Cell proliferation-inducing gene 21 protein; guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1 sequence 1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; guanine nucleotide binding protein beta polypeptide 2-like 1; guanine nucleotide binding protein related; guanine nucleotide binding protein, beta 2, related sequence 1; guanine nucleotide binding protein, beta-2, related sequence 1; Guanine nucleotide-binding protein subunit beta-2-like 1; Guanine nucleotide-binding protein subunit beta-like protein 12.3; HLC-7; Human lung cancer oncogene 7 protein; lung cancer oncogene 7; OTTHUMP00000223870; OTTHUMP00000223891; OTTHUMP00000223893; OTTHUMP00000223900; OTTHUMP00000223901; OTTHUMP00000223902; OTTHUMP00000223930; OTTHUMP00000223931; p205; proliferation-inducing gene 21; protein homologous to chicken B complex protein, guanine nucleotide binding; rack-1; Receptor for activated C kinase; Receptor of activated protein C kinase 1; Receptor of activated protein kinase C 1; Small ribosomal subunit protein RACK1
Gene Aliases: AL033335; GB-like; Gnb2-rs1; GNB2L1; H12.3; HLC-7; HLC7; p205; PIG21; RACK1
UniProt ID: (Mouse) P68040, (Human) P63244
Entrez Gene ID: (Mouse) 14694, (Human) 10399
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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