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Proteintech

RAF1 Monoclonal Antibody (1D6A1)

5 References

View all (76) c-Raf antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)

Application

RAF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

RAF1 Antibody (66592-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAF1 antibody (Product # 66592-1-IG, Clone: 1D6A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (Blue).

RAF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

66592-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:150-1:600

Immunocytochemistry (ICC/IF)

1:800

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1D6A1

Immunogen

RAF1 Fusion Protein Ag25423 (1-59 aa encoded by BC018119)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEHIQGAWK TISNGFGFKD AVFDGSSCIS PTIVQQFGYQ RRASDDGKLT DPSKTSNTIR (1-59 aa encoded by BC018119 )

Target Information

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway.

Scientific reports

Tang L,Guo C,Li X,Zhang B,Huang L

Mon Apr 10 00:00:00 UTC 2023

Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling.

World journal of gastrointestinal oncology

Zheng W,Shen GL,Xu KY,Yin QQ,Hui TC,Zhou ZW,Xu CA,Wang SH,Wu WH,Shi LF,Pan HY

Sat Jan 15 00:00:00 UTC 2022

Exosomal DNAJB11 promotes the development of pancreatic cancer by modulating the EGFR/MAPK pathway.

Cellular & molecular biology letters

Liu P,Zu F,Chen H,Yin X,Tan X

Sat Oct 08 00:00:00 UTC 2022

4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis.

Cancer cell international

Li W,Huang BS,Xiong YY,Yang LJ,Wu LX

Tue Jan 18 00:00:00 UTC 2022

Huai Qi Huang-induced Apoptosis via Down-regulating PRKCH and Inhibiting RAF/MEK/ERK Pathway in Ph⁺ Leukemia Cells.

Current medical science

Xu WF,Wang ZJ,Li K,Shen YQ,Lu K,Lv XY,Wen YX,Jin RM

Wed Apr 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: C-Raf proto-oncogene, serine/threonine kinase; cRaf; Craf1 transforming; EC 2.7.11.1; kinase Raf1; murine sarcoma 3611 oncogene 1; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; sarcoma 3611 oncogene; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine le

Gene Aliases: 6430402F14Rik; AA990557; BB129353; c-Raf; CMD1NN; CRAF; Craf1; D830050J10Rik; NS5; RAF; Raf-1; RAF1; v-Raf

UniProt ID: (Human) P04049, (Mouse) Q99N57

Entrez Gene ID: (Human) 5894, (Mouse) 110157

Function(s)

protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding kinase activity small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding metal ion binding protein heterodimerization activity nucleotide binding receptor signaling protein activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding non-receptor serine/threonine protein kinase

Process(es)

MAPK cascade activation of MAPKK activity response to hypoxia stimulatory C-type lectin receptor signaling pathway protein phosphorylation apoptotic process signal transduction activation of adenylate cyclase activity heart development cell proliferation negative regulation of cell proliferation cell differentiation platelet activation thyroid gland development negative regulation of protein complex assembly positive regulation of peptidyl-serine phosphorylation ion transmembrane transport somatic stem cell population maintenance regulation of Rho protein signal transduction insulin secretion involved in cellular response to glucose stimulus response to muscle stretch wound healing regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process intermediate filament cytoskeleton organization regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway thymus development face development death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of cell motility cellular glucose homeostasis positive regulation of protein phosphorylation phosphorylation intracellular signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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