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            • Primary Antibodies ›
            • RAG2 Antibodies

            Invitrogen

            RAG2 Polyclonal Antibody

            Advanced Verification
            View all (17) RAG2 antibodies
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            Cite RAG2 Polyclonal Antibody

            • Antibody Testing Data (2)
            • Advanced Verification (1)
            Application
            RAG2 Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            RAG2 Antibody (PA5-110264) in WB

            Western blot analysis of RAG2 in extracts of various cell lines. Samples were incubated with RAG2 Polyclonal antibody (Product # PA5-110264) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
            View Product

            RAG2 Antibody in Western Blot (WB)
            RAG2 Antibody in Western Blot (WB)
            RAG2 Antibody

            Product Details

            PA5-110264

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:2,000
            -

            ELISA (ELISA)

            1 µg/mL
            -
            Product Specifications

            Species Reactivity

            Human, Mouse

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Recombinant fusion protein containing a sequence corresponding to amino acids 351-527 of human RAG2 (NP_0005272)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.72 mg/mL

            Purification

            Affinity Chromatography

            Storage buffer

            PBS, pH 7.3, with 50% glycerol

            Contains

            0.05% ProClin 300

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2855675

            Product Specific Information

            Immunogen sequence: AEDDTNEEQT TFTNSQTSTE DPGDSTPFED SEEFCFSAEA NSFDGDDEFD TYNEDDEEDE SETGYWITCC PTCDVDINTW VPFYSTELNK PAMIYCSHGD GHWVHAQCMD LAERTLIHLS AGSNKYYCNE HVEIARALHT PQRVLPLKKP PMKSLRKKGS GKILTPAKKS FLRRLFD

            Target Information

            RAG2 (V(D)J recombination-activating protein 2) is a core component of the RAG complex - a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity) and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: 1) first a nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends. 2) hairpin coding ends and 2 blunt, 5'-phosphorylated ends are created. The chromatin structure plays an essential rold in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and hair pinning expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin. This prevents accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. Mutations affecting the gene can results in combined cellular and humoral immune defects with granulomas, severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive, and Omenn syndrome.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: RAG 2; RAG-2; recombinase activating gene 2; recombination activating protein 2; V(D)J recombination-activating protein 2

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            Gene Aliases: RAG-2; RAG2

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            UniProt ID: (Human) P55895, (Mouse) P21784

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            Entrez Gene ID: (Human) 5897, (Mouse) 19374

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            Function(s)

            DNA binding chromatin binding phosphatidylinositol-4,5-bisphosphate binding phosphatidylinositol-3,4,5-trisphosphate binding zinc ion binding methylated histone binding phosphatidylinositol binding phosphatidylinositol-3,4-bisphosphate binding ubiquitin protein ligase activity phosphatidylinositol-3,5-bisphosphate binding protein binding metal ion binding

            Process(es)

            B cell lineage commitment pre-B cell allelic exclusion B cell homeostatic proliferation T cell lineage commitment protein ubiquitination covalent chromatin modification B cell differentiation T cell differentiation in thymus V(D)J recombination positive regulation of organ growth DNA recombination chromatin modification T cell differentiation
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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