Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
RAGE Polyclonal Antibody
Promotions
View available promotion(s)
Promo Code: P5877545 Don't miss out: buy 3, only pay for 2 From June 2-13 take advantage of our 3 for 2 promotion. Whether you are restocking essentials or trying something new, now is a great time to get more for your money!. Learn more
Promo Code: RPUZZ25 Stock up on essentials to piece your discovery together Until June 27, save up to $650 and get an exclusive lab-themed hidden-object puzzle. Learn more
Application
FIGURE: 1 / 3
RAGE Antibody (PA5-48057) in WB
Western blot was performed using Anti-RAGE Polyclonal Antibody (Product # PA5-48057) and 45, 55 kDa bands corresponding to RAGE was observed in Mouse Lung, Rat Lung but not in Mouse Heart, Mouse Skeletal Muscle which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Lung (Lane 1), Rat Lung (Lane 2), Mouse Heart (Lane 3) and Mouse Skeletal Muscle (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB... View More
Product Details
PA5-48057
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Published species
Rat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant canine RAGE Asp25-Val339
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576242
Product Specific Information
In sandwich ELISAs, approximately 0.5% cross-reactivity with recombinant human RAGE is observed and less than 0.1% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
The Receptor for Advanced Glycation End-products (RAGE) is a gene located on human chromosome 6p21.3, encoding a transmembrane receptor belonging to the immunoglobulin superfamily. RAGE is expressed in various tissues, with significant levels in the lungs, and plays a crucial role in cellular signaling and inflammation. As a receptor, RAGE binds multiple ligands, including advanced glycation end-products (AGEs), amyloid-ß peptide, high mobility group box 1 (HMGB1), and S100/calgranulin proteins, facilitating diverse pathological processes like inflammation, cancer progression, and neurodegeneration. The interaction between RAGE and its ligands triggers intracellular signaling pathways such as NF-?B activation, leading to inflammatory responses and oxidative stress. In the context of chronic diseases like diabetes, Alzheimer's, and cardiovascular diseases, RAGE is a critical mediator, linking metabolic disturbance to cellular dysfunction. Therapeutic targeting of RAGE signaling is under investigation, aiming to mitigate its contribution to inflammatory and degenerative diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: advanced glycation end product receptor; advanced glycation end-product receptor; RAGE-4 ORF3; RAGE/AGER; receptor for advanced glycation endproducts; receptor of advanced glycosylation end products of proteins; immunoglobulin superfamily; MHC class II; MHC class III; sRAGE
Gene Aliases: RAGE
Entrez Gene ID: (Dog) 403168
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support