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Invitrogen
RALA Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
RALA Antibody (PA5-79910) in ICC/IF
Immunofluorescence analysis of RALA was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RALA Polyclonal Antibody (Product # PA5-79910) at 5µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution ... View More
Product Details
PA5-79910
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RALA (165-203aa DKVFFDLMREIRARKMEDSKEKNGKKKRKSLAKRIRERC).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747025
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: -ral simian leukemia viral oncogene homolog A (ras related); MGC48949; ral-A protein; Ras family small GTP binding protein RALA; ras related GTP binding protein A; RAS-like protein A; RAS-like, family 1; Ras-related protein Ral-A; v-ral simian leukemia viral oncogene homolog A (ras related)
Gene Aliases: 3010001O15Rik; AW322615; RAL; Ral-a; RALA; Rasl1
UniProt ID: (Human) P11233, (Mouse) P63321, (Rat) P63322
Entrez Gene ID: (Human) 5898, (Mouse) 56044, (Rat) 81757
cytokinesis
neural tube closure
exocytosis
chemotaxis
signal transduction
Ras protein signal transduction
viral process
regulation of exocytosis
actin cytoskeleton reorganization
positive regulation of filopodium assembly
membrane raft localization
membrane organization
cell cycle
small GTPase mediated signal transduction
cell division
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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