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Application
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RALA Antibody (MA5-50740) in IHC (P)
Immunohistochemistry of RALA in paraffin-embedded human colon carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with RALA recombinant monoclonal antibody (Product # MA5-50740) with a dilution of 1:200 for 1 hour at ... View More
Product Details
MA5-50740
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE54-12
Immunogen
Recombinant protein within Human RALA aa 107-206/206.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3092886
Product Specific Information
Positive control: LOVO cell lysate, HCT116 cell lysate, HT-29 cell lysate, SW480 cell lysate, 293 cell lysate, human kidney tissue lysate, human colon tissue, human colon carcinoma tissue, human kidney tissue, HUVEC.
Predicted band size: 24 kDa
Subcellular Location: Cell membrane, Mitochondrion.
Target Information
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC48949; Ras family small GTP binding protein RALA; ras related GTP binding protein A; RAS-like protein A; Ras-related protein Ral-A; v-ral simian leukemia viral oncogene homolog A (ras related)
Gene Aliases: RAL; RALA
UniProt ID: (Human) P11233
Entrez Gene ID: (Human) 5898
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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