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Invitrogen

RAN Polyclonal Antibody

Advanced Verification

View all (27) RAN antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (1)

Application

RAN Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

RAN Antibody (PA5-17652) in ICC/IF

Immunofluorescence analysis of RAN was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAN Polyclonal Antibody (Product # PA5-17652) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilutio... View More

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RAN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAN siRNA and decrease in signal intensity was observed in western blot application using Anti-RAN Polyclonal Antibody (Product # PA5-17652). {KD}

Product Details

PA5-17652

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Bovine, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

20 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10981803

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other small GTPases of the Ras family.

Target Information

Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Androgen receptor-associated protein 24; ARA25; CG1404-PA; CG1404-PB; CG1404-PC; CG1404-PD; GM14354p; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; LD32416p; LD40852p; member RAS oncogene family; OK/SW-cl.81; OTTHUMP00000166078; OTTHUMP00000166102; ran; ran GTP-binding protein; Ran GTPase; RAN, member RAS oncogene family; Ran-PA; Ran-PB; Ran-PC; Ran-PD; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein

Gene Aliases: AAF30287; ARA24; CG1404; Dmel\CG1404; Dmel_CG1404; dran; Gsp1; I79_024397; l(1)G0075; OK/SW-cl.81; Q9VZ23; RAN; ran-1; Ran10A; RanGTP; RanTC4; Rasl2-8; TC4; XELAEV_18007741mg; xran

UniProt ID: (Human) Q9CWI7, (Bovine) Q3T054, (Rat) P62828, (Fruit fly) Q9NH14, (Mouse) P62827, (Xenopus) P52301

Entrez Gene ID: (Human) 5901, (Bovine) 540457, (Rat) 84509, (Fruit fly) 44072, (Chinese hamster) 100769066, (Mouse) 19384, (Xenopus) 108716335

Function(s)

chromatin binding transcription coactivator activity GTPase activity protein binding GTP binding GDP binding protein domain specific binding protein complex binding poly(A) RNA binding dynein intermediate chain binding androgen receptor binding importin-alpha family protein binding pre-miRNA binding pre-miRNA transporter activity cadherin binding involved in cell-cell adhesion magnesium ion binding nucleotide binding small GTPase

Process(es)

ribosomal large subunit export from nucleus ribosomal small subunit export from nucleus protein import into nucleus, translocation DNA metabolic process tRNA export from nucleus protein export from nucleus mitotic spindle organization mitotic nuclear division signal transduction small GTPase mediated signal transduction spermatid development viral process hippocampus development actin cytoskeleton organization androgen receptor signaling pathway gene silencing by RNA positive regulation of protein binding cellular protein complex localization pre-miRNA export from nucleus positive regulation of transcription, DNA-templated cell division cellular response to mineralocorticoid stimulus intracellular transport of virus cell-cell adhesion protein localization to nucleolus mitotic sister chromatid segregation protein import into nucleus GTP metabolic process snRNA import into nucleus microtubule cytoskeleton organization nucleocytoplasmic transport metabolic process cellular protein localization ribosomal subunit export from nucleus establishment of mitotic spindle orientation meiotic spindle organization intracellular protein transport regulation of mitotic cell cycle regulation of protein localization nuclear pore complex assembly transport cell cycle protein transport regulation of protein binding

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